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Figure 10 | SpringerPlus

Figure 10

From: Development of a novel cellular model of Alzheimer’s disease utilizing neurosphere cultures derived from B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J embryonic mouse brain

Figure 10

Detection of intracellular Aβ peptides by CDIC. (A) Fixed and permeabilized adherent cells from neurosphere cultures were either treated with formic acid (FA) or left untreated followed by immunostaining with 6E10 antibody. Detection was done using Alexa 594 labeled secondary antibody. (B) Quantification of fluorescence intensity indicates that Tg+ve NS lines show significant increased signal over Tg-ve lines in both the treatments. FA treatment significantly increased signal in Tg+ve NS lines (NS1 and NS3) compared to non-treated counterparts suggesting the possible presence of intracellular Aβ peptides (non-parametric one-way ANOVA, n = 3, H = 1909.061 and p < 0.001).

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