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Figure 1 | SpringerPlus

Figure 1

From: Development of a novel cellular model of Alzheimer’s disease utilizing neurosphere cultures derived from B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J embryonic mouse brain

Figure 1

Development of neurosphere (NS) cultures from B6C3-Tg( APPswe,PSEN1dE9 )85Dbo/J E15 embryos. (A) Cells from four mouse embryos (E15) were isolated and grown in non-treated tissue culture flasks to form neurospheres (NS1-NS4). (B) Genotyping of NS1-NS4 for huAPPswe (350-bp) and huPSEN1dE9 (608-bp) transgenes by PCR. (C) Expression of nestin in NS1-4 lines by immunofluorocytochemistry. (D) Cell type analysis of nestin expression in NS1-NS4 lines is represented as histograms of mean ± standard deviation (ANOVA, n = 4, F = 0.174, p = 0.912).

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