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Table 4 Effects of sequence context of the ATG initiation codon on translational efficiency of EGFP expressed in PSG

From: Effect of ATG initiation codon context motifs on the efficiency of translation of mRNA derived from exogenous genes in the transgenic silkworm, Bombyx mori

 

Line no.

EGFP protein (μg/larva) (A)

EGFP mRNA (×10−7) (pmol/ng) (B)

rp49 mRNA (×10−7) (pmol/ng) (C)

EGFP/rp49 mRNA (B/C)

Translational efficiency (A/{B/C})

Fold efficiency (%)

NC

1

12.9 ± 0.8

4.20

3.95

1.06

12.1

 

-EGFP

2

9.9 ± 0.3

1.73

3.54

0.49

20.2

 
 

3

12.6 ± 0.7

0.48

3.60

0.14

93.0

 
 

Average

11.8

2.14

3.70

0.56

41.8

100

PC

1

80.6 ± 15.3

1.35

2.93

0.46

175.5

 

-EGFP

2

104.8 ± 24.2

2.14

3.65

0.59

178.1

 
 

Average

92.7

1.75

3.29

0.52

176.8

423

AAT

1

25.5 ± 3.6

1.64

3.23

0.51

50.3

 

-EGFP-EGFP

2

53.5 ± 15.8

0.84

3.50

0.24

220.5

 
 

Average

39.5

1.24

3.37

0.37

135.4

324

Ser1

1

94.4 ± 10.4

1.53

3.52

0.44

216.9

 

-EGFP

2

106.2 ± 34.7

1.15

3.09

0.37

284.9

 
 

Average

100.3

1.34

3.31

0.40

250.9

600

  1. Values obtained from each line and averages of lines are shown. The fold efficiency is indicated as the efficiency of the average of NC equal to 100.