Effect of ATG initiation codon context motifs on the efficiency of translation of mRNA derived from exogenous genes in the transgenic silkworm, Bombyx mori

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Table 3 Effects of sequence context of the ATG initiation codon on translational efficiency of EGFP expressed in MSG

	Line no.	EGFP protein (μg/larva) (A)	EGFP mRNA (×10⁻⁷) (pmol/ng) (B)	rp49 mRNA (×10⁻⁷) (pmol/ng) (C)	EGFP/rp49 mRNA (B/C)	Translational efficiency (A/{B/C})	Fold efficiency (%)
NC	1	31.4 ± 5.8	70.8	7.83	9.05	3.47
-EGFP	2	16.8 ± 2.4	44.1	7.26	6.07	2.76
	3	12.6 ± 1.7	49.9	7.70	6.48	1.95
	Average	20.3	54.9	7.60	7.23	2.73	100
PC	1	130.2 ± 9.9	19.8	6.96	2.85	45.7
-EGFP	2	158.9 ± 12.1	29.6	6.71	4.40	36.1
	Average	144.5	24.7	6.84	3.61	40.9	1,498
AAT	1	76.8 ± 7.2	48.7	9.04	5.39	14.2
-EGFP	2	89.0 ± 12.9	34.4	8.14	4.23	21.0
	Average	82.9	41.6	8.59	4.84	17.6	645
Ser1	1	276.3 ± 36.4	40.8	8.14	5.01	55.1
-EGFP	2	261.4 ± 18.4	37.3	7.76	4.80	54.4
	Average	268.9	39.0	7.95	4.91	54.8	2,007

Values obtained from each line and averages of lines are shown. The fold efficiency is indicated as the efficiency of the average of NC equal to 100.