Table 1 Effects of the ATG initiation codon sequence context of Photinus luciferase constructs on Photinus luciferase expression in BmN4 cells
Context name | Photinus luciferase activity (×104) (A) | Renilla luciferase activity (×102) (B) | Photinus/Renilla(A/B) | Fold activity (%) |
|---|---|---|---|---|
NC-Luc | 8.3 ± 1.1 | 4.26 ± 0.7 | 196.5 ± 15.0 | 100 |
PC-Luc | 88.3 ± 26.6 | 4.34 ± 0.5 | 2,008.7 ± 393.1 | 1,022 |
7N3P-Luc | 55.9 ± 20.4 | 4.23 ± 0.5 | 1,307.5 ± 393.6 | 665 |
7P3N-Luc | 56.8 ± 10.2 | 4.49 ± 0.8 | 1,272.5 ± 184.9 | 648 |
AAT-Luc | 38.7 ± 27.4 | 4.44 ± 0.6 | 834.6 ± 539.9 | 425 |
AGT-Luc | 39.4 ± 6.7 | 4.28 ± 0.6 | 941.6 ± 252.9 | 479 |
CAA-Luc | 15.0 ± 2.7 | 4.80 ± 0.4 | 316.8 ± 84.3 | 161 |
CGA-Luc | 11.1 ± 2.4 | 4.82 ± 0.4 | 228.2 ± 30.8 | 116 |
Ser1-Luc | 45.0 ± 24.1 | 4.68 ± 0.6 | 928.2 ± 373.2 | 472 |
Vert3-Luc | 25.5 ± 3.1 | 4.66 ± 0.3 | 547.9 ± 61.0 | 279 |
Vert9-Luc | 36.9 ± 6.6 | 4.81 ± 0.3 | 775.4 ± 173.8 | 395 |
atgA-Luc | 24.3 ± 16.9 | 4.47 ± 0.5 | 550.6 ± 413.8 | 280 |
atgC-Luc | 34.3 ± 2.1 | 4.56 ± 0.5 | 759.1 ± 102.1 | 386 |
atgT-Luc | 27.9 ± 12.5 | 4.58 ± 0.8 | 594.0 ± 171.2 | 302 |