Figure 3

Expression of EGFP in the transgenic silkworm. a. Stemmata of the fifth instar larva. UAS-EGFP constructs with NC, PC, AAT, and Ser1 sequence contexts of the initiation codon were marked with a 3 × P3-EGFP marker construct allowing the expression of EGFP in the stemmata. The GAL4 driver construct under the control of the sericin 1, fibroin H chain, or 30 k protein gene promoter was linked with a marker construct, 3 × P3-DsRed, allowing the expression of DsRed in the stemmata. The stemmata of a larva harboring PC-EGFP, Ser1-GAL4, or both PC-EGFP and Ser1-GAL4 are shown. The images were taken under white light or under a fluorescence microscope equipped with an EGFP or DsRed filter. Arrows indicate the stemmata with EGFP or DsRed fluorescence. Scale bar: 1 mm. b. EGFP expression in the MSG, PSG, and the fat body of the transgenic silkworm with different GAL4 driver constructs. MSG, PSG, and the fat body on the sixth day of the fifth instar are shown. Images were taken under a fluorescence microscope equipped with an EGFP filter. Expression of UAS-EGFP with different context motifs was only observed in silkworms with GAL4 drivers. The numbers above the tissue photographs indicate the transgenic silkworm lines. Scale bar: 10 mm.