Optimization of protein production by Micrococcus luteus for exploring pollutant-degrading uncultured bacteria

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Table 3 Central composite design for optimizing significant variables for protein production

Run	Mineral solution (ml/L)	Lithium L-lactate (g/L)	Initial pH	Incubation time (h)	Protein yield (mg/L)
Run	Mineral solution (ml/L)	Lithium L-lactate (g/L)	Initial pH	Incubation time (h)	Actual value	Predicted value
1	1.5	7.5	8.25	72	20.92 ± 0.45	20.84
2	2	5	7.5	48	22.94 ± 0.23	22.4
3	1.5	7.5	8.25	120	1.53 ± 0.33	1.88
4	1	5	7.5	48	21.21 ± 0.12	20.16
5	2	5	7.5	96	13.93 ± 0.38	12.01
6	2	5	9	48	13.11 ± 0.66	13.32
7	2	10	9	96	6.71 ± 0.49	7.31
8	1.5	7.5	9.75	72	7.81 ± 0.61	7.51
9	1	5	7.5	96	9.22 ± 0.36	9.25
10	1.5	2.5	8.25	72	11.71 ± 0.76	13.05
11	2	10	9	48	17.01 ± 0.43	15.59
12	1	5	9	96	4.70 ± 0.56	6.38
13	1.5	7.5	8.25	72	20.31 ± 0.57	20.84
14	1	10	7.5	96	12.08 ± 0.30	11.41
15	1.5	7.5	8.25	72	21.03 ± 0.49	20.84
16	1.5	7.5	8.25	72	21.18 ± 1.16	20.84
17	1	10	9	96	9.00 ± 0.36	8.16
18	1	10	9	48	15.50 ± 0.89	16.97
19	1.5	7.5	8.25	24	19.58 ± 0.54	21.08
20	1.5	7.5	8.25	72	20.97 ± 0.43	20.84
21	2	5	9	96	7.32 ± 0.53	5.84
22	2	10	7.5	48	27.19 ± 0.35	25.05
23	1	10	7.5	48	23.02 ± 0.34	23.12
24	0.5	7.5	8.25	72	15.74 ± 0.34	15.19
25	2.5	7.5	8.25	72	14.17 ± 0.60	16.57
26	1.5	7.5	8.25	72	20.61 ± 0.83	20.84
27	2	10	7.5	96	13.79 ± 0.74	13.86
28	1	5	9	48	15.83 ± 1.44	14.38
29	1.5	7.5	6.75	72	17.69 ± 0.50	19.84
30	1.5	12.5	8.25	72	16.98 ± 0.31	17.48