Figure 5

Inhibition of TE- or Rg1-induced AMPK activation in EA.hy926 cells. Confluent EA.hy926 cells were pretreated with wortmannin (PI3K/Akt inhibitor, 10 μM), compound C (AMPK inhibitor, 10 μM), or L-NAME (NOS inhibitor, 100 μM) for 30 min, then treated with 150 μg/mL TE (A) or 26 μM Rg1 (B) for 10 min. Representative western blots of intracellular AMPK or ß–actin are presented. Quantitative analysis of optical density of AMPK (normalized to the density of ß–actin) is shown at the bottom. Each bar represents mean ± SD, from three separate wells per condition. ^#P < 0.05 compared with TE stimulation without inhibitor.