Figure 4

Inhibition of TE- or Rg1-induced eNOS activation in EA.hy926 cells. Confluent EA.hy926 cells were pretreated with wortmannin (PI3K/Akt inhibitor, 10 μM), compound C (AMPK inhibitor, 10 μM), or L-NAME (NOS inhibitor, 100 μM) for 30 min, then treated with 150 μg/mL TE (A) or 26 μM Rg1 (B) for 10 min. Representative western blots of intracellular eNOS or ß–actin are presented. Quantitative analysis of optical density of eNOS (normalized to the density of ß–actin) is shown at the bottom. Each bar represents mean ± SD, from three separate wells per condition. ^*P < 0.05 compared with control. ^#P < 0.05 compared with TE stimulation without inhibitor.