Rhodomycin analogues from Streptomyces purpurascens: isolation, characterization and biological activities

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Table 4 A detailed description of sugar and aglycone substituent groups of all the isolated anthracyclines obtained from S. purpurascens

Fraction	Aglycones		Sugars			Compound predicted
Fraction	Rf^adetected	Aglycone	Rf^b	Color	Sugar detected	Compound predicted
A	0.27	α₂ –RMN	0.56	grayish blue	2-deoxy-L- fucose	α_2-Rhodomycin II
B	0.45	β RMN	0.56	grayish blue	L-Rhodosamine	Rhodomycin
B	0.45	β RMN	0.56	grayish blue	2-deoxy-L- fucose	Rhodomycin
C	0.79	ε RMN	0.56	grayish blue	2-deoxy-L- fucose	Epelmycin B
D	0.67	Aklavinone	0.12	sky blue	L-Rhodosamine	Aclacinomycin A
D	0.67	Aklavinone	0.56	grayish blue	2-deoxy-L- fucose	Aclacinomycin A
E	0.45	β-RMN	0.12	sky blue	L-Rhodosamine	Rhodomycin A or B
E	0.45	β-RMN	0.56	grayish blue	2-deoxy-L- fucose	Rhodomycin A or B
F	0.59	γ-iso RMN	0.12	sky blue	L-Rhodosamine	Obelmycin
F	0.59	γ-iso RMN	0.56	grayish blue	2-deoxy-L- fucose	Obelmycin
G	0.27	α₂ –RMN	0.12	sky blue	L-Rhodosamine	Alldimycin B
G	0.27	α₂ –RMN	0.56	grayish blue	2-deoxy-L- fucose	Alldimycin B

a: CHCl₃ : MeOH (15:1), b: Butanol: acetic acid: water (4:1:1).
Identification of the sugars and aglycones were done by comparing their Rf with that of the standards under similar TLC solvent conditions.