Figure 4
(i) Preparative TLC- For the partial purification of antimicrobial products preparative thin layer chromatography was performed. The crude extract got resolved into seven well separated colored bands when developed in the Chloroform: Methanol: 25% aqueous NH3 (85:14:1) solvent system. According to the Rf value, each fraction was named from A to D. (ii) Antimicrobial activity of the isolated fractions against B. subtilis and S. aureus determined by agar well diffusion assay. Zone of inhibition associated with each fraction against respective test organisms is depicted.