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Table 1 Quantitative overview of recombinant P5βR2 purification in the early- (0.1) or mid-log- (0.6) phase culture

From: Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature

Culture density 4°C/72 h 15°C/18 h
  P5βR2 (mg) Total protein in cell lysate (mg) Yield (%) P5βR2 (mg) Total protein in cell lysate (mg) Yield (%)
OD600 = 0.1 3.6 ± 0.2 12.1 ± 1.3 30 3.5 ± 0.3 21.4 ± 1.9 16
OD600 = 0.6 1.4 ± 0.2 20.8 ± 3.4 8 3.8 ± 0.4 23.0 ± 2.8 17
  1. Escherichia coli cultures were incubated at low temperatures (4°C or 15°C) for the indicated time period. Data are the mean values (±SD) from three separate 150 ml expression cultures.