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Table 1 Quantitative overview of recombinant P5βR2 purification in the early- (0.1) or mid-log- (0.6) phase culture

From: Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature

Culture density

4°C/72 h

15°C/18 h

 

P5βR2 (mg)

Total protein in cell lysate (mg)

Yield (%)

P5βR2 (mg)

Total protein in cell lysate (mg)

Yield (%)

OD600 = 0.1

3.6 ± 0.2

12.1 ± 1.3

30

3.5 ± 0.3

21.4 ± 1.9

16

OD600 = 0.6

1.4 ± 0.2

20.8 ± 3.4

8

3.8 ± 0.4

23.0 ± 2.8

17

  1. Escherichia coli cultures were incubated at low temperatures (4°C or 15°C) for the indicated time period. Data are the mean values (±SD) from three separate 150 ml expression cultures.