Culture density | 4°C/72 h | 15°C/18 h | ||||
---|---|---|---|---|---|---|
P5βR2 (mg) | Total protein in cell lysate (mg) | Yield (%) | P5βR2 (mg) | Total protein in cell lysate (mg) | Yield (%) | |
OD600 = 0.1 | 3.6 ± 0.2 | 12.1 ± 1.3 | 30 | 3.5 ± 0.3 | 21.4 ± 1.9 | 16 |
OD600 = 0.6 | 1.4 ± 0.2 | 20.8 ± 3.4 | 8 | 3.8 ± 0.4 | 23.0 ± 2.8 | 17 |