Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas

SpringerPlus

Table 4 A summary of in vitro immunomodulatory screening of the endophytic fungal extracts by the lymphocyte proliferation assay

S.No.	Test Extract	Dose μg/ml	Immunomodulatory activity (% lymphocyte proliferation) Inhibition (↓) /stimulation (↑)
	Cont		T -Cell	B-Cell
		-	-	-
1.	K1	1	34↓*	54↓**
		10	31↓	22↓
2.	K2	1	15	46↑
		10	12	49↑
3.	K4	1	31↑	40↑
		10	42↑	57↑
4.	K7	1	40↓**	37↓*
		10	25↓	46↓**
5.	DEF3	1	32↑	65↑**
		10	37↑	48↑
6.	DEF4	1	34↓	51↓
		10	34↓	44↓
7.	Art	1	28↑	47↑
		10	33↑	22↑
8.	Art3	1	44↓*	38↓*
		10	37↓*	52↓**
9.	Art4	1	25↓	51↓
		10	40↓**	45↓**
10.	FEF2	1	56↑	40↑*
		10	29↑	47↑**
11.	FEF4	1	57↑**	11
		10	62↑**	18
12.	NF2	1	14↑	32↑*
		10	28↑	45↑
13.	PR1	1	51↑**	19↑
		10	34↑	14↑
14.	PR2	1	15↑	48↑**
		10	28↑	34↑
15.	PR3	1	48↑	43↑
		10	28↑	54↑**
16.	PR4	1	25↑	15↑
		10	16↑	44↑*
Betamethasone		0.005	46↓	34↓
Levamisole		0.05	29↑	43↑

16 extracts demonstrated significant immune-modulation. Levamisole (as stimulator) and Betamethasone (as an immunesuppressor) were used as positive controls. Data are presented as % inhibition or stimulation of sets of independent experiments. * P<0.05 and ** P<0.01 represent significant difference compared with cells treated with Levamisole and Betamethasone.