Table 1 The list of endophytes isolated in this study, their host plants and the summary of BLAST results, showing the coverage of the sequences and sequence similarities with the most closely related organisms (Altschul et al. 1997)
No. | Plant host; Voucher no. | Endophytes isolated (GenBank Acc. no. of the ITS sequence) | Query coverage | % sequence similarity | Organism with the highest sequence identity, GenBank Acc. no. |
|---|---|---|---|---|---|
1. | Picrorhiza kurroa; RRL(H)17772 | PR1 ( JQ769230 ) | 100 | 95 | Chaetomium globosum, GQ365152.1 |
| Â | Â | PR2 ( JQ769231) | 99 | 99 | Valsa sordida, HQ420239.1 |
| Â | Â | PR3 ( JQ769232 ) | 98 | 93 | Thielavia subthermophila, JN390827.1 |
| Â | Â | PR4 ( JQ769233 ) | 100 | 99 | Diaporthe phaseolorum, EF488429.1 |
2. | Cannabis sativa; RRL(H)18255 | CN1 ( JQ769234 ) | 100 | 98 | Alternaria alternata, JN038476.1 |
| Â | Â | CN2 ( JQ769235 ) | 100 | 99 | Schizophyllum commune, EU030374.1 |
| Â | Â | CN3 ( JQ769236 ) | 99 | 97 | Alternaria sp. JN689942.1 |
| Â | Â | CN4 ( JQ769237 ) | 100 | 96 | Alternaria brassicae, JF439450.1 |
3. | Withania somnifera; RRL(H)22006 | WEF1 ( JQ769238 ) | 100 | 99 | Gibberella moniliformis, GQ168841.1 |
| Â | Â | WEF2 ( JQ769239 ) | 100 | 99 | Cochliobolus lunatus, GQ328851.1 |
| Â | Â | WEF3 ( JQ769240 ) | 100 | 99 | Fusarium sp., EU236709.1 |
| Â | Â | WEF4 ( JQ769241 ) | 100 | 99 | Fusarium equiseti, EU595566.1 |
| Â | Â | WEF5 ( JQ769242 ) | 100 | 98 | Gibberella moniliformis, JF499680.1 |
| Â | Â | WEF7 ( JQ769243 ) | 100 | 98 | Hypoxylon fragiforme, JN564001.1 |
| Â | Â | WEF8 ( JQ769244 ) | 100 | 99 | Nigrospora sphaerica, HQ608030.1 |
| Â | Â | WEF9 ( JQ769245 ) | 100 | 94 | Cercophora caudata, AY999135.1 |
| Â | Â | WEF10 ( JQ769246 ) | 100 | 95 | Cladosporium cladosporioides, JN618353.1 |
4. | Rauwolfia serpentina; RRL(H)19053 | RSL1 ( JQ769247) | 100 | 97 | Alternaria brassicae, JF439438.1 |
| Â | Â | RSL2 ( JQ769248 ) | 100 | 99 | Alternaria sp. HQ596520.1 |
| Â | Â | RSL3 ( JQ769249 ) | 100 | 98 | Cladosporium cladosporioides, GQ221853.1 |
| Â | Â | RSL4 ( JQ769250 ) | 100 | 98 | Fusarium proliferatum, X94171.1 |
| Â | Â | RSL5 ( JQ769251 ) | 100 | 97 | Alternaria brassicae, JF439444.1 |
| Â | Â | RSS2 ( JQ769252 ) | 100 | 98 | Alternaria alternata, JN618076.1 |
| Â | Â | RSS3 ( JQ769253 ) | 100 | 97 | Diaporthe helianthi, AJ312356.1 |
| Â | Â | RSS4 ( JQ769254 ) | 100 | 98 | Fusarium proliferatum, HQ022511.1 |
| Â | Â | RSS5 ( JQ769255 ) | 100 | 99 | Alternaria sp. , HQ875381.1 |
| Â | Â | RSS6 ( JQ769256 ) | 100 | 97 | Alternaria alternata, JN618076.1 |
| Â | Â | RSS7 ( JQ769257 ) | 100 | 97 | Alternaria sp. , GU934499.1 |
| Â | Â | RSS9 ( JQ769258 ) | 100 | 97 | Lasiodiplodia theobromae, GQ502453.1 |
| Â | Â | RSR1 ( JQ769259 ) | 100 | 98 | Glomerella acutata, HM575269.1 |
5. | Cedrus deodara; RRL(H)1832) | DEF1 ( JQ769260 ) | 100 | 98 | Sordaria humana, EU918705.1 |
| Â | Â | DEF2 ( JQ769261 ) | 100 | 100 | Alternaria alternaria, JN618076.1 |
| Â | Â | DEF3 ( JQ769262 ) | 100 | 93 | Talaromyces trachyspermus, AY533702.1 |
| Â | Â | DEF4 ( JQ769263 ) | 100 | 99 | Cochliobolus spicifer, JN192387.1 |
| Â | Â | DEF5 ( JQ769264 ) | 99 | 98 | Scleroconidioma sphagnicola, FR837912.1 |
6. | Abies pindrow; RRL(H)21551 | FEF2 ( JQ769265 ) | 99 | 99 | Daldinia fissa, AM292038.1 |
| Â | Â | FEF3 ( JQ769266 ) | 99 | 96 | Penicillium oxalicum, GU078430.1 |
| Â | Â | FEF4 ( JQ769267 ) | 99 | 98 | Polyporus arcularius, AF516524.1 |
| Â | Â | FEF5 ( JQ769268 ) | 98 | 95 | Apiosordaria otanii, GQ922524 |
7. | Pinus roxburgii; RRL(H)15011 | K1 ( JQ769269 ) | 99 | 99 | Petriella sp. EU315012.1 |
| Â | Â | K2 ( JQ769270 ) | 100 | 97 | Bipolaris tetramera, HM195268.1 |
| Â | Â | K4 ( JQ769271 ) | 100 | 94 | Trichophaea abundans EU715596 |
| Â | Â | K6 ( JQ769272 ) | 100 | 95 | Penicillium expansum, FJ770072.1 |
| Â | Â | K7 ( JQ769273 ) | 99 | 99 | Ulocladium sp. JF311922.1 |
8. | Nothapodytes nimmoniana; RRL(H)20702 | NF1 ( JQ769274 ) | 100 | 98 | Phomopsis sp. FJ441623.1 |
| Â | Â | NF2 ( JQ769275 ) | 100 | 93 | Petriella setifera, AY882356.1 |
9. | Platanus orientalis; RRL(H)19697 | CH2 ( JQ769276 ) | 100 | 99 | Fusarium tricinctum, HM776425.1 |
| Â | Â | CH3 ( JQ769277 ) | 100 | 100 | Fusarium solani, EU314965.1 |
| Â | Â | CH5 ( JQ769278 ) | 100 | 99 | Fusarium sp., EU589150.1 |
| Â | Â | CH6 ( JQ769279 ) | 100 | 100 | Gibberella sp., FJ196601.1 |
10. | Artemisia annua; RRL(H)18128) | Art ( JQ769280 ) | 100 | 100 | Fusarium tricinctum, AB369452.1 |
| Â | Â | Art1 ( JQ769281 ) | 100 | 99 | Fusarium flocciferum, GQ505465.1 |
| Â | Â | Art2 ( JQ769282 ) | 98 | 98 | Gibberella avenacea, HM036596.1 |
| Â | Â | Art3 ( JQ769283 ) | 100 | 99 | Sordaria superba, EU551188.1 |
| Â | Â | Art4 ( JQ769284 ) | 100 | 100 | Fusarium redolens, JF311916.1 |
| Â | Â | Art5 ( JQ769285 ) | 100 | 99 | Chaetomium sp., HQ914898.1 |
| Â | Â | Art6 ( JQ769286 ) | 100 | 100 | Alternaria alternata, GU073112.1 |
| Â | Â | Art7 ( JQ769287 ) | 100 | 99 | Alternaria alternata, JN618076.1 |
| Â | Â | Art8 ( JQ769288 ) | 100 | 99 | Chaetomium globosum, GQ365152.1 |
| Â | Â | Art9 ( JQ769289 ) | 100 | 98 | Alternaria sp., HQ914883.1 |
| Â | Â | Art10 ( JQ769290 ) | 100 | 99 | Alternaria brassicae, JF439439.1 |
| Â | Â | Art11 ( JQ769291 ) | 100 | 99 | Alternaria sp., JF694748.1 |
| Â | Â | Art12 ( JQ769292 ) | 100 | 99 | Alternaria sp., GU934499.1 |
| Â | Â | Art14 ( JQ769293 ) | 100 | 98 | Alternaria brassicae, JF439444.1 |
| Â | Â | Art15 ( JQ769294 ) | 100 | 99 | Alternaria citri, DQ339104.1 |
| Â | Â | Art17 ( JQ769295 ) | 100 | 98 | Alternaria brassicae, JF439444.1 |
| Â | Â | Art18 ( JQ769296 ) | 100 | 100 | Paraphoma sp., FJ903342.1 |
| Â | Â | Art19 ( JQ769297 ) | 98 | 93 | Alternaria tenuissima, HQ343444.1 |
| Â | Â | Art20 ( JQ769298 ) | 100 | 98 | Alternaria arborescens, AY154706.1 |
| Â | Â | Art23 ( JQ769299 ) | 88 | 92 | Alternaria alternata, JF311960.1 |
| Â | Â | Art25 ( JQ769300 ) | 100 | 99 | Alternaria sp., JF694748.1 |
| Â | Â | Art36 ( JQ769301 ) | 100 | 96 | Gibberella avenacea, EU255804.1 |