|
A
|
|---|
|
Amino acid
|
No lactic acid bacteria
|
L. lactis subsp. lactis JCM 5805a
|
L. reuteri JCM 1112
|
L. gasseri JCM 1131
|
L. brevis JCM 1170
|
|---|
|
μMb
|
% D
c
|
μM
|
% D
|
μM
|
% D
|
μM
|
% D
|
μM
|
% D
|
|---|
|
D-Asp
|
32.1
|
2.7
|
74.8
|
6.4
|
150.2
|
16.7
|
225.0
|
16.3
|
316.7
|
24.4
|
|
D-Ser
|
7.7
|
0.5
|
13.3
|
1.1
|
13.8
|
1.0
|
34.9
|
1.7
|
20.4
|
1.1
|
|
D-Gln
|
− d
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-His
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Thr
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Arg
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Ala
|
33.3
|
1.1
|
402.5
|
13.8
|
286.0
|
8.2
|
1,410.6
|
41.4
|
934.4
|
24.5
|
|
D-Tyr
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Val
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Met
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Trp
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Phe
|
N.D.e
|
−
|
6.3
|
0.4
|
N.D.
|
−
|
5.7
|
0.4
|
5.5
|
0.3
|
|
D-aIlo-Ile
|
−
|
−
|
−
|
−
|
41.6
|
2.9
|
−
|
−
|
−
|
−
|
|
D-Leu
|
−
|
−
|
−
|
−
|
31.4
|
1.2
|
−
|
−
|
N.D.
|
−
|
|
D-Glu
|
13.3
|
0.4
|
22.9
|
0.8
|
246.3
|
8.2
|
101.9
|
3.2
|
88.0
|
70.7
|
|
D-Asn
|
13.9
|
1.8
|
12.6
|
1.8
|
11.5
|
6.3
|
16.4
|
5.5
|
14.2
|
2.1
|
|
B
|
|
Amino acid
|
L. salivarius
JCM 1231
a
|
L. otakiensis
JCM 15040
|
L. kisonensis
JCM 15041
|
L. rapi
JCM 15042
|
|
μM
b
|
%
D
c
|
μM
|
%
D
|
μM
|
%
D
|
μM
|
%
D
|
|
D-Asp
|
118.3
|
26.3
|
128.7
|
22.0
|
120.4
|
22.9
|
110.1
|
11.5
|
|
D-Ser
|
13.6
|
1.0
|
14.1
|
1.1
|
24.0
|
1.4
|
12.4
|
0.9
|
|
D-Gln
|
− d
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-His
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Thr
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Arg
|
35.9
|
3.7
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Ala
|
537.8
|
19.0
|
336.0
|
9.9
|
576.2
|
16.2
|
309.6
|
8.7
|
|
D-Tyr
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Val
|
−
|
−
|
42.9
|
2.1
|
−
|
−
|
−
|
−
|
|
D-Met
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Trp
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Phe
|
N.D.e
|
−
|
10.7
|
0.8
|
N.D.
|
−
|
N.D.
|
−
|
|
D-aIlo-Ile
|
−
|
−
|
141.3
|
9.8
|
−
|
−
|
−
|
−
|
|
D-Leu
|
N.D.
|
−
|
194.0
|
5.9
|
N.D.
|
−
|
N.D.
|
−
|
|
D-Glu
|
118.7
|
4.3
|
54.7
|
1.7
|
161.0
|
5.7
|
65.5
|
2.0
|
|
D-Asn
|
11.9
|
2.5
|
10.9
|
2.4
|
14.0
|
3.0
|
4.9
|
0.8
|
-
aDetails of the sample preparation are provided in the Methods.
-
bConcentrations cited are smaller one among the amino acid concentrations determined using NAC and NBC as chiral regents.
-
cRelative percentages of D-amino acids were calculated as 100 × [D-amino acid]/[D-amino acid + L-amino acid].
-
d−, not detected.
-
eN. D., not determined. The D-amino acid peak was confirmed, but the concentration of the D-amino acid was too low to be determined.
