|
Amino acid
|
Sample 1a
|
Sample 2
|
Sample 3
|
Sample 4
|
Sample 5
|
|---|
|
μMb
|
% D
c
|
μM
|
% D
|
μM
|
% D
|
μM
|
% D
|
μM
|
% D
|
|---|
|
D-Asp
|
68.2
|
0.3
|
68.8
|
0.4
|
69.0
|
0.4
|
99.4
|
0.4
|
599.0
|
2.7
|
|
D-Ser
|
− d
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
22.7
|
0.7
|
|
D-Gln
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-His
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Thr
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Arg
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
37.2
|
65.7
|
|
D-Ala
|
24.6
|
0.1
|
29.5
|
0.2
|
41.1
|
0.3
|
28.1
|
0.1
|
1,962.4
|
10.7
|
|
D-Tyr
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Val
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
81.6
|
8.0
|
|
D-Met
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
32.3
|
21.6
|
|
D-Trp
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
|
D-Phe
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
89.7
|
3.2
|
|
D-aIlo-Ile
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
−
|
420.9
|
38.6
|
|
D-Leu
|
−
|
−
|
−
|
−
|
N. D. e
|
N. D.
|
−
|
−
|
285.0
|
37.3
|
|
D-Glu
|
67.6
|
0.2
|
66.8
|
0.2
|
64.7
|
0.2
|
81.7
|
0.2
|
209.1
|
0.7
|
|
D-Asn
|
78.0
|
0.5
|
71.4
|
0.6
|
62.9
|
0.5
|
71.7
|
0.4
|
71.0
|
0.5
|
-
aDetails of samples 1–5 are provided in the Methods.
-
bConcentrations cited are smaller one among the amino acid concentrations determined using NAC and NBC as chiral regents.
-
cRelative percentages of D-amino acids were calculated as 100 × [D-amino acid]/[D-amino acid + L-amino acid].
-
d−, not detected.
-
eN. D., not determined. The D-amino acid peak was confirmed, but the concentration of the D-amino acid was too low to be determined.
