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Figure 3

From: Mutant LV476-7AA of A-subunit of Enterococcus hirae V1-ATPase: High affinity of A3B3 complex to DF axis and low ATPase activity

Figure 3

ATPase activities of mutant A 3 B 3 DF (V 1 ) complexes (containing mutation in A- and/or B-subunits) of E. hirae V-ATPase at various ATP concentrations. ATPase assay was started by the addition of 4 μg proteins. Experimental details were described in “Materials and methods”. (A) ATPase activities depending on the various concentrations of ATP were shown. (B) Lineweaver-Burk plots of the ATPase activities for the calculation of K m and V max. Filled diamonds, A(LV476-7AA)3B3DF; filled circles, A(LV476-7AA)3B(L389A)3DF; and filled triangles, wild-type A3B3DF.

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