Cryotolerance strategies of Pseudomonads isolated from the rhizosphere of Himalayan plants

Bisht, Shekhar Chandra; Joshi, Gopal Kishna; Haque, Shafiul; Mishra, Pankaj Kumar

doi:10.1186/2193-1801-2-667

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Table 3 Quantitative analysis (HPLC) of intracellular amino acids’ content of Pseudomonas strains at 4°C and 28°C growth temperatures

From: Cryotolerance strategies of Pseudomonads isolated from the rhizosphere of Himalayan plants

Amino acid (pico mole mg^-1cell dry wt.^-1)*	*Pseudomonas* strains
	*P. lurida* NPRs3		*P. lurida* NPRp15		*Pseudomonas* sp. PPERs23		*P. putida* PGRs4		*Pseudomonas* sp. PGERs17		*P. fluorescens* PPRs4
	At 4°C	At 28°C	At 4°C	At 28°C	At 4°C	At 28°C	At 4°C	At 28°C	At 4°C	At 28°C	At 4°C	At 28°C
Aspartic acid	65.8 ± 1.5b	39.9 ± 0.9a	33.1 ± 0.7b	20.3 ± 0.5a	14.1 ± 0.3b	11.1 ± 0.2a	333.9 ± 7.5b	166.5 ± 3.7a	51.7 ± 1.2b	27.5 ± 0.6a	18.0 ± 0.5b	5.9 ± 0.3a
Proline	4355 ± 98b	1997 ± 45a	7317 ± 164b	3751.2 ± 84a	1880 ± 42b	1229.7 ± 27a	1467 ± 33b	1352.2 ± 30a	4733.3 ± 106b	1738.2 ± 39a	1613 ± 26b	586.5 ± 13a
Cysteine	32.8 ± 0.7a	89.7 ± 2b	439.4 ± 9.9b	78.2 ± 1.8a	67.8 ± 1.5	-	89.9 ± 2b	52.5 ± 1.2a	30.8 ± 0.7b	17.4 ± 0.4a	71.8 ± 1.8b	1.5 ± 0.1a
Serine	485.0 ± 10.9b	57.9 ± 1.3a	28.1 ± 0.6b	23.0 ± 0.5a	12.0 ± 0.4a	21.6 ± 0.5b	14.2 ± 0.3b	7.7 ± 0.2a	13.6 ± 0.3a	19.0 ± 0.4b	13.1 ± 0.6b	2.9 ± 0.1a
Glutamic acid	11.8 ± 0.3a	26.2 ± 0.6b	14.8 ± 0.3a	16.1 ± 0.4a	8.8 ± 0.2b	3.9 ± 0.1a	26.1 ± 0.6a	46.5 ± 1.1b	28.7 ± 0.6b	7.4 ± 0.2a	5.9 ± 0.3a	8.0 ± 0.4b
Glycine	662.0 ± 14.9b	347.5 ± 7.8a	274.5 ± 6.2a	370.7 ± 8.3b	350.8 ± 7.9a	184.7 ± 4.2b	137.7 ± 3.1b	121.5 ± 2.7a	351.2 ± 7.9a	533.3 ± 12b	288.8 ± 6.2b	272.9 ± 6.1a
Histidine	97.6 ± 2.2a	192.6 ± 4.3b	148.2 ± 3.3b	134.4 ± 3a	50.8 ± 1.1a	63.0 ± 1.4b	20.9 ± 0.5b	4.6 ± 0.1a	103.9 ± 2.3b	55.6 ± 1.3a	73.9 ± 1.8b	65.9 ± 1.5a
Arginine	158.1 ± 3.6b	27.6 ± 0.6a	12.9 ± 0.3b	9.8 ± 0.2a	4.5 ± 0.1b	1.2 ± 0.1a	58.2 ± 1.3a	81.1 ± 1.8b	8.2 ± 0.2a	9.4 ± 0.2a	7.7 ± 0.3b	4.4 ± 0.1a
Threonine	190.5 ± 4.3a	1084.3 ± 24b	9194.7 ± 20b	3757.1 ± 84a	5143.0 ± 115a	6722.1 ± 151b	6510.4 ± 146b	1795 ± 40.4a	3003.8 ± 67.6b	714.0 ± 16a	6479.2 ± 57b	3890.4 ± 87a
Alanine	57.5 ± 1.3a	167.73.8b	107.9 ± 2.4b	94.1 ± 2.1a	38.3 ± 0.9a	39.7 ± 0.9a	104.6 ± 2.4a	890.9 ± 20b	91.0 ± 2b	66.3 ± 1.5b	46.5 ± 1.3a	50.1 ± 1.1b
Tyrosine	30.2 ± 0.7a	46.5 ± 1.2b	39.2 ± 0.9b	19.6 ± 0.4a	10.7 ± 0.3a	9.5 ± 0.4a	6.5 ± 0.1a	3874.7 ± 87b	16.8 ± 0.4b	0.8a	14.0 ± 0.5a	166.8 ± 3.8b
Valine	27.1 ± 0.6a	64.9 ± 1.5b	40.0 ± 1.9b	23.2 ± 0.5a	9.5 ± 0.2a	8.3 ± 0.3a	3.1 ± 0.1a	26.4 ± 0.6b	17.0 ± 0.4b	6.5 ± 0.1a	14.1 ± 0.4b	7.0 ± 0.2a
Methionine	11.6 ± 0.3a	37.50.8b	18.5 ± 0.4a	18.9 ± 0.4a	5.5 ± 0.1a	7.3 ± 0.2b	13.2 ± 0.3b	10.0 ± 0.2a	12.8 ± 0.3a	11.4 ± 0.3a	10.3 ± 0.3a	10.2 ± 0.2a
Lysine	40.0 ± 0.9a	41.6 ± 0.9a	58.5 ± 1.3a	153.7 ± 3.5b	9.8 ± 0.5a	20.2 ± 0.5b	303.5 ± 6.8a	301.2 ± 6.8a	69.1 ± 1.6a	120.9 ± 2.7b	5.1 ± 0.2a	11.3 ± 0.3b
Isoleucine	16.3 ± 0.4a	54.4 ± 1.2b	778.3 ± 17.5b	15.4 ± 0.3a	0.2a	0.4a	49.8 ± 1.1b	27.9 ± 0.6a	-	-	34.5 ± 0.7b	5.5 ± 0.2a
Leucine	9.7 ± 0.2a	18.5 ± 0.4b	103.6 ± 2.3b	12.3 ± 0.4a	6.1 ± 0.2a	5.1 ± 0.1a	78.9 ± 1.8b	22.6 ± 0.5a	9.1 ± 0.3b	6.7 ± 0.2a	73.1 ± 1.3b	9.9 ± 0.3a
Phenylalanine	21.2 ± 0.5a	81.6 ± 1.8b	38.6 ± 0.9b	23.9 ± 0.5a	6.6 ± 0.3a	5.4 ± 0.2a	118.9 ± 2.7b	4.9 ± 0.1a	8.3 ± 0.2b	4.9 ± 0.1a	130.1 ± 3.3b	5.4 ± 0.1a

*(-): Not detected.
Note: All values are mean of three (n = 3) experiments, followed by ± Standard deviation.
Letters (a,b) in the same column for each Pseudomonas strain indicate significant difference at 4°C and 28°C incubation temperatures.

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