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Table 2 Quantitative analysis of intracellular sugars and polyols content of Pseudomonas strains by HPLC

From: Cryotolerance strategies of Pseudomonads isolated from the rhizosphere of Himalayan plants

Pseudomonas strains

Temperature

Content (μg mg-1cell dry weight-1)*

Sugar’ content

Polyols content

Raffinose

Sucrose

Trehalose

Glucose

Mannitol

Sorbitol

P. lurida NPRs3

28°C

8.92 ± 1.52a

-

-

-

2.73 ± 0.52a

-

4°C

19.79 ± 2.26b

-

1.89 ± 0.21

-

2.23 ± 0.34a

9.36 ± 1.61

P. lurida NPRp15

28°C

-

-

-

105.4 ± 3.9

43.23 ± 3.63

-

4°C

41.69 ± 3.39b

-

3.19 ± 0.81

-

-

-

Pseudomonas sp. PPERs23

28°C

3.18 ± 1.28a

-

16.46 ± 1.62b

-

7.12 ± 1.07b

22.06 ± 1.96b

4°C

13.41 ± 1.76b

-

2.49 ± 0.48a

-

0.55 ± 0.01a

3.80 ± 1.56a

P. putida PGRs4

28°C

4.38 ± 0.97a

-

0.44 ± 0.09a

-

-

3.17 ± 1.01a

4°C

11.25 ± 1.13b

-

3.14 ± 0.84b

-

3.92 ± 0.66

50.49 ± 3.68b

Pseudomonas sp. PGERs17

28°C

1.56 ± 0.54a

-

-

-

-

0.91 ± 0.03a

4°C

9.90b

-

3.02 ± 1.6

-

-

4.32 ± 0.98b

P. fluorescens PPRs4

28°C

6.76 ± 1.38

-

-

-

-

-

4°C

14.22 ± 1.74

3.33 ± 1.25

-

-

-

-

  1. *(-): Not detected.
  2. Note: All values are mean of three (n = 3) experiments, followed by ± Standard deviation.
  3. Letters (a,b) in the same column for each Pseudomonas strain indicate significant difference at 4°C and 28°C incubation temperatures.