Figure 6

Effects of mast cell stabilization on leukocyte counts in MSU crystal-induced acute inflammation. To confirm the role of mast cell in gout, mast cell stabilizer ketotifen was examined in gout model. Rats were divided into five groups of six: Group I, negative control group; Group II, rats were injected MSU crystal (5 mg/rat) into air pouch; Group III-V, rats were given ketotifen 0.1, 1, and 10 mg/kg orally 30 min before MSU crystal. The same dose of ketotifen was then given intravenously as a bolus 15 min before MSU crystal. Leukocyte numbers in pouch lavage were determined 12 h after MSU. Data are means ± SD (n = 6). Significant differences between groups were analyzed using one-way ANOVA. Different letters above the bars indicate significant (P < 0.05) differences.