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Table 3 Calibration data, dynamic range and human milk reproducibility of FAA (n = 6)

From: Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk

Free amino acid Linear regression data Dynamic range CV (%)b
  r2 pmol/μLa  
L-Histidine 0.9998 0.10 – 133.3 2.8
Taurine 0.9993 0.05 – 100 1.8
L-Serine 0.9994 0.05 – 26.7 2.3
L-Glutamine 0.9982 0.05 – 100 8.3
L-Arginine 0.9956 0.1 – 6.7 10.0
Glycine 0.9991 0.05 – 26.7 2.3
L-Aspartic acid 0.9980 0.05 – 26.7 2.9
L-Glutamic acid 0.9986 0.05 – 200 1.6
L-Citrulline 0.9974 0.05 – 5 8.6
L-Threonine 0.9990 0.05 – 26.7 2.8
L-Alanine 0.9953 0.05 – 53.3 2.2
L-Proline 0.9984 0.2 – 53.3 7.1
L-Cystine 0.9948 0.05 – 53.3 15.5
L-Tyrosine 0.9958 0.05 – 26.7 2.7
L-Lysine 0.9954 0.1 – 6.7 8.5
L-Methionine 0.9973 0.05 – 26.7 2.7
L-Valine 0.9973 0.05 – 26.7 2.5
L-Isoleucine 0.9947 0.05 – 13.3 4.4
L-Leucine 0.9968 0.05 – 13.3 2.9
L-Phenylalanine 0.9982 0.05 – 26.7 7.0
L-Tryptophan 0.9986 0.05 – 5 7.5
  1. aassay concentration of AQC amino acid derivatives, 1 μL injected.
  2. bcoefficient variation of reproducibility, calculated on 6 independent milk samples tested on 2 days with 2 operators.