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Table 1 Gradient elution used for free amino acid analysis (Armenta et al. 2010 ), Eluent A was 10% AccQ•TagUltra concentrate solvent A in deionized water and eluent B was 100% AccQ•TagUltra solvent B

From: Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk

Chromatographic gradient program over UPLC-MS/MS analysis time (10 min)
Time (min) Mobile phase Mobile phase Flow rate
A (%) B (%) (mL/min)
0 99.9 0.1 0.7
0.54 99.9 0.1 0.7
5.74 90.0 10.0 0.7
7.74 78.8 21.2 0.7
8.04 40.4 59.6 0.7
8.64 40.4 59.6 0.7
8.73 99.9 0.1 0.7
10.00 99.9 0.1 0.7