Chromatographic gradient program over UPLC-MS/MS analysis time (10 min) | |||
---|---|---|---|
Time (min) | Mobile phase | Mobile phase | Flow rate |
A (%) | B (%) | (mL/min) | |
0 | 99.9 | 0.1 | 0.7 |
0.54 | 99.9 | 0.1 | 0.7 |
5.74 | 90.0 | 10.0 | 0.7 |
7.74 | 78.8 | 21.2 | 0.7 |
8.04 | 40.4 | 59.6 | 0.7 |
8.64 | 40.4 | 59.6 | 0.7 |
8.73 | 99.9 | 0.1 | 0.7 |
10.00 | 99.9 | 0.1 | 0.7 |