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Table 1 Gradient elution used for free amino acid analysis (Armenta et al. 2010 ), Eluent A was 10% AccQ•TagUltra concentrate solvent A in deionized water and eluent B was 100% AccQ•TagUltra solvent B

From: Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk

Chromatographic gradient program over UPLC-MS/MS analysis time (10 min)

Time (min)

Mobile phase

Mobile phase

Flow rate

A (%)

B (%)

(mL/min)

0

99.9

0.1

0.7

0.54

99.9

0.1

0.7

5.74

90.0

10.0

0.7

7.74

78.8

21.2

0.7

8.04

40.4

59.6

0.7

8.64

40.4

59.6

0.7

8.73

99.9

0.1

0.7

10.00

99.9

0.1

0.7