A reliable and effective method of DNA isolation from old human blood paper cards

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Table 3 Success rates of amplifying caspase-12 C125T SNP (rs497116) by PCR and a Taqman genotyping assay using four different reagents

	PCR successful rate	PCR successful rate	Taqman genotyping successful rate
	(system 1)	(system 2)
Method	(success#/total) (%)	(success#/total) (%)	(success#/total) (%)
DN131	(92/105) (87.6)	(75/105) (71.4)	(74/105) (70.5)
Tris–HCl	(94/105) (89.5)	(99/105) (96.1)	(86/105) (81.9)
TE	(77/105) (73.3)	(92/105) (88.5)	(74/105) (70.5)
H₂O	(76/105) (72.4)	(82/105) (78.1)	(79/105) (79.1)

System 1 and system 2 are two different PCR amplification systems we used and described in Materials and methods.