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Table 3 Success rates of amplifying caspase-12 C125T SNP (rs497116) by PCR and a Taqman genotyping assay using four different reagents

From: A reliable and effective method of DNA isolation from old human blood paper cards

  PCR successful rate PCR successful rate Taqman genotyping successful rate
  (system 1) (system 2)  
Method (success#/total) (%) (success#/total) (%) (success#/total) (%)
DN131 (92/105) (87.6) (75/105) (71.4) (74/105) (70.5)
Tris–HCl (94/105) (89.5) (99/105) (96.1) (86/105) (81.9)
TE (77/105) (73.3) (92/105) (88.5) (74/105) (70.5)
H2O (76/105) (72.4) (82/105) (78.1) (79/105) (79.1)
  1. System 1 and system 2 are two different PCR amplification systems we used and described in Materials and methods.