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Table 2 Quantity and quality of DNA isolated from old human blood paper cards using different reagents

From: A reliable and effective method of DNA isolation from old human blood paper cards

 

DNA quantity

DNA quality

Methods

(ng/ml)

A260/A280

A260/A230

 

(min, max)

(min, max)

(min, max)

DN 131

103.3 ± 8.6

2.07 ± 0.6

0.06 ± 0.01

(−64.9, 566.9)

(−6.64, 52.18)

(−0.03, 0.37)

Tris–HCl

190.0 ± 6.1

1.5 ± 0.01

0.5 ± 0.01

(62.7, 397.7)

(1.17, 1.72)

(0.35, 0.75)

TE

183.5 ± 6.4

2.08 ± 0.1

1.06 ± 0.1

(54.2, 444.8)

(1.30, 8.90)

(0.4, 6.25)

H2O

203.5 ± 9.0

1.5 ± 0.01

0.4 ± 0.01

 

(38.8, 489.2)

(1.23, 1.68)

(0.22, 0.82)

  1. In each space, values are represented as mean ± standard error. The minimum (min) and maximum (max) values are shown in parenthesis.