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Table 1 Procedure for DNA isolation from blood paper card

From: A reliable and effective method of DNA isolation from old human blood paper cards

Step Procedure
1 Cut a blood paper card spot to 4 small pieces of equal size
2 Transfer paper card pieces to an eppendorf tube with an isolation solution
3 Rotate the tubes overnight at 4°C
4 Heat the tubes at 95°C for 10 min
5 Centrifuge at 10,000 g at the room temperature for 10 min
  The supernatant can be used directly for PCR