Figure 3

PCR amplification of a DNA fragment of caspase-12 gene covering C125T SNP (rs497116) using 105 DNA samples isolated by different reagents (DN131, Tris–HCl, TE and water) with two different PCR amplification systems. A. A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 1, which was detailed in Materials and methods. PCR bands were visualized under UV light after ethidium bromide staining; B. PCR band density quantification by Image J for Figure 3A. *, p < 0.05; **, p < 0.01, compared to DN131; C. A representative image of PCR amplified bands separated by a 2% agarose gel electrophoresis for system 2, which was detailed in Materials and methods; D. PCR band density quantification by Image J for Figure 3C. ***, p < 0.001, compared to DN131.