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Figure 2 | SpringerPlus

Figure 2

From: A reliable and effective method of DNA isolation from old human blood paper cards

Figure 2

Bland-Altman Plots of DNA concentrations, A260/A280 and A260/A230 ratios between the two methods using DN131 and using our solutions (Tris–HCl, TE or water). A. Bland-Altman Plot of DNA concentrations between DN131 and Tris–HCl. Correlation R = 0.38 (p < 0.0001), slope = − 0.76 (p < 0.001), intercept = 193.27 (p < 0.0001); B. Bland-Altman Plot of DNA concentrations between DN131 and TE. Correlation R = 0.28 (p < 0.004), slope = − 0.46 (p = 0.004), intercept = 145.59 (p < 0.0001); C. Bland-Altman Plot of DNA concentrations between DN131 and H2O. Correlation R = 0.05 (p = 0.55), slope = 0.32 (p = 0.96), intercept = 59.65 (p = 0.99); D. Bland-Altman Plot of A260/A280 ratios between DN131 and Tris–HCl. Correlation R = 0.99 (p < 0.0001), slope = − 2.0 (p < 0.0001), intercept = 2.95 (p < 0.0001); E. Bland-Altman Plot of A260/A280 ratios between DN131 and TE. Correlation R = 0.97 (p < 0.0001), slope = − 1.97 (p < 0.0001), intercept = 3.51 (p < 0.0001); F. Bland-Altman Plot of A260/A280 ratios between DN131 and H2O. Correlation R = 0.99 (p < 0.0001), slope = −20 (p < 0.0001), intercept = 2.91 (p < 0.0001); G. Bland-Altman Plot of A260/A230 ratios between DN131 and Tris–HCl. Correlation R = 0.06 (p = 0.57), slope = 0.64 (p = 0.99), intercept = 0.27 (p = 0.99); H. Bland-Altman Plot of A260/A230 ratios between DN131 and TE. Correlation R = 0.99 (p < 0.0001), slope = 1.99 (p < 0.0001), intercept = −0.12 (p = 0.007); I. Bland-Altman Plot of A260/A230 ratios between DN131 and H2O. Correlation R = 0.47 (p < 0.0001), slope = 0.97 (p < 0.0001), intercept = 0.13 (p = 0.007).

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