Figure 2
From: 5-Aminolevulinic acid enhances cancer radiotherapy in a mouse tumor model
Intracellular ROS level viability of ALA and X-ray treatment. Intracellular ROS level of B16-BL6 mouse melanoma cells with various concentrations of ALA and different X-ray doses in vitro. ALA was added 24 h before X-ray irradiation. Before X-ray irradiation, CellROX™ Deep Red Reagent was added at a final concentration of 10 μM to the cells. After X-ray irradiation, plates were incubated for 30 min at 37 degree. Subsequently, medium was removed and the cells were washed with PBS. The resulting fluorescence was measured using a microplate reader. Data are given as mean ± SD (n = 4). An asterisk indicates significant difference compared to without ALA. *P < 0.05, **P < 0.01 for ALA treatment vs. the untreated control.