Table 2 % similarity of 463 bp sequence of 16S rRNA gene of type sp. ( Bacillus subtilis ) with 16S rRNA sequences of different AEFB strains (downloaded from NCBI), primer sequences in these AEFB strains, presence and absence of restriction enzyme site and position of specific fragment in AEFB strains

1

Alicyclobacillus sacchari

AB264020

AATCC GTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

913-1374

2

Alicyclobacillus acidiphilus

AB076660

AATCC GTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%-

913-1374

3

Alicyclobacillus acidoterrestris

AB042057

AATCC GTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

910-1371

4

Alicyclobacillus hesperidum

AJ133633

AATCC GTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

884-1345

5

Alicyclobacillus fastidiosus

AB264021

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

910-1371

6

Alicyclobacillus vulcanalis

AY425985

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

894-1355

7

Alicyclobacillus sendaiensis

AB084128

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

885-1346

8

Alicyclobacillus contaminans

AB264026

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

925-1386

9

Alicyclobacillus acidocaldarius subsp. acidocaldarius (Type sp)

AJ496806

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

87%+

902-1363

10

Alicyclobacillus aeris

FM179383

AATCC GTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

911-1372

11

Alicyclobacillus pomorum

AB089840

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

911-1372

12

Alicyclobacillus disulfidooxidans

AB089843

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

85%+

911-1372

13

Alicyclobacillus tolerans

Z21979

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

87%-

906-1365

14

Alicyclobacillus ferrooxydans

EU137838

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

913-1374

15

Alicyclobacillus cycloheptanicus

AB042059

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

911-1372

16

Alicyclobacillus macrosporangiidus

AB264025

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

87%-

927-1388

17

Alicyclobacillus kakegawensis

AB264022

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

86%+

924-1385

18

Alicyclobacillus shizuokensis

AB264024

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

87%+

924-1385

19

Alicyclobacillus herbarius

AB042055

AATC CGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

87%+

924-1385

20

Alicyclobacillus pohliae

AJ564766

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

88%+

904-1363

21

Alicyclobacillus tolerans

Z21979

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

87%-

906-1365

22

Amphibacillus sediminis

AB243866

AATACGTTCCCGGGTC TT CTGAAACTCAAAA GAATTGACG

96%-

928-1386

23

Amphibacillus jilinensis,

FJ169626

AATACGTTCCCGGGTC TT CTGAAACTCAAAA GAATTGACG

95%-

948-1406

24

Amphibacillus tropicus

AF418602

AATACGTTCCCGGGTC TT CTGAAACTCAAAA GAATTGACG

95%-

905-1362

25

Amphibacillus fermentum

AF418603

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

93%-

910-1368

26

Amphibacillus xylanus, type sp.

D82065

AATACGTTCCCGGGTC TT CTGAAACTCAAAA GAATTGACG

94%-

948-1406

27

Aneurinibacillus aneurinilyticus type sp.

X94194

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

91%-

903-1369

28

Aneurinibacillus migulanus

X94195

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

90%-

903-1359

29

Aneurinibacillus danicus

AB112725

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

91%-

903-1354

30

Aneurinibacillus thermoaerophilus

X94196

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

92%-

904- 1361

31

Aneurinibacillus terranovensis

AJ715385

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

91%-

897-1353

32

Brevibacillus centrosporus

D78458

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

917-1377

33

Brevibacillus choshinensis

AB112713

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

894-1354

34

Brevibacillus reuszeri

AB112715

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

894-1354

35

Brevibacillus parabrevis

AB112714

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

894-1354

36

Brevibacillus brevis type sp.

AB271756

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

896-1356

37

Brevibacillus formosus

AB112712

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

894-1354

38

Brevibacillus agri

AB112716

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

895-1355

39

Brevibacillus limnophilus

AB112717

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

909-1369

40

Brevibacillus invocatus

AF378232

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

896-1356

41

Brevibacillus panacihumi

EU383033

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

902-1362

42

Brevibacillus borstelensis

AB112721

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

894-1354

43

Brevibacillus ginsengisoli

AB245376

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

873-1333

44

Brevibacillus laterosporus

D16271

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

896-1356

45

Brevibacillus fluminis

EU375457

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

896-1356

46

Brevibacillus levickii

AJ715378

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

897-1357

47

Brevibacillus thermoruber

Z26921

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

915-1376

48

Gracilibacillus lacisalsi

DQ664540

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

94%+

933-1393

49

Gracilibacillus thailandensis

FJ182214

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

94%+

942-1402

50

Gracilibacillus saliphilus

EU784646

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

94%+

917-1377

51

Gracilibacillus orientalis

AM040716

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

93%+

931-1391

52

Gracilibacillus dipsosauri

AB101591

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

95%+

923-1383

53

Gracilibacillus ureilyticus

EU709020

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

95%+

923-1383

54

Gracilibacillus boraciitolerans

AB197126

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

94%+

935-1395

55

Gracilibacillus halotolerans type sp.

AF036922

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

94%+

934-1394

56

Gracilibacillus halophilus

EU135704

CTGAAACTCAAAA GAATTGACG AATACGTTCCCGGGCCTT

94%+

924-1384

57

Paenibacillus polymyxa type sp.

D16276

AATACGTTCCCGGGT CTT CTGAAACTCAAAGGAATTGACG

90%-

913-1375

58

Paenibacillus antarcticus

AJ605292

AATACGTTCCCGGGT CTT CTGAAACTCAAAGGAATTGACG

90%-

915-1374

59

Paenibacillus macquariensis subsp. macquariensis

X60625

AATACGTTCCCGGGT CTT CTGAAACTCAAAGGAATTGACG

90%-

935-1394

60

Paenibacillus macquariensis subsp. defensor

AB360546

AATACGTTCCCGGGT CTT CTGAAACTCAAAGGAATTGACG

90%+

936-1395

61

Paenibacillus glacialis

EU815294

AATACGTTCCCGGGT CTT CTGAAACTCAAAGGAATTGACG

91%-

934-1393

62

Virgibacillus pantothenticus type sp.

D16275

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

95%-

919-1375

63

Virgibacillus proomii

AJ012667

CTGAAACTCAAAAGA ATTGACG AATACGTTCCCGGGTC TT

95%-

916-1372

64

Virgibacillus salexigens

Y11603

CTGAAACTCAAAAG AATTNACG AATACGTTCCCGGGCCTT

95%+

921-1379

65

Virgibacillus marismortui

AJ009793

AATACGTTCCCGGGCCTT CTGAAACTCAAAAG AATTGACG

95%+

947-1407

66

Virgibacillus salarius

AB197851

AATACGTTCCCGGGCCTT CTGAAACTCAAAA GAATTGACG

95%+

949-1409

67

Virgibacillus olivae

DQ139839

AATACGTTCCCGGGCCTT CTGAAACTCAAAAG AATTGACG

95%+

948-1409

68

Virgibacillus halodenitrificans

AY543169,

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

926-1386

69

Virgibacillus koreensis

AY616012

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

97%+

926-1386

70

Virgibacillus halophilus

AB243851

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

94%+

926-1386

71

Virgibacillus sediminis

AY121430

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

96%-

946-1406

72

Virgibacillus xinjiangensis

DQ664543

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

96%-

894-1354

73

Virgibacillus chiguensis

EF101168

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

96%-

919-1375

74

Virgibacillus dokdonensis

AY822043

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGT CTT

96%-

927-1383

75

Virgibacillus carmonensis

AJ316302

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

925-1385

76

Virgibacillus necropolis

AJ315056

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

925-1385

77

Virgibacillus arcticus

EF675742

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

809- 1269

78

Virgibacillus byunsanensis

FJ357159

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

923-1383

79

Virgibacillus salinus

FM205010

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

932-1392

80

Virgibacillus subterraneus

FJ746573

AATACGTTCCCGGC CCTT CTGAAACTCAAAAG AATTGACG

91%+

905-1362

81

Virgibacillus kekensis

AY121439

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

945-1405

82

Geobacillus stearothermophilus type sp.

AB021196,

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

912-1376

83

Geobacillus kaustophilus

X60618

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

933-1390

84

Geobacillus lituanicus

AY044055

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

933-1397

85

Geobacillus thermoleovorans

Z26923

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

918-1382

86

Geobacillus thermocatenulatus

AY608935

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

940-1404

87

Geobacillus jurassicus

AY312404

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

915-1379

88

Geobacillus uzenensis

AF276304

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

907-1370

89

Geobacillus subterraneus

AF276306

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

931-1395

90

Geobacillus thermodenitrificans

AY608961

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

939-1409

91

Geobacillus debilis

AJ564616

AATACGTTCT CGGGCCTT CTGAAACTCAAAGGAATTGACG

91%-

936-1398

92

Geobacillus toebii

AF326278

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

910-1374

93

Geobacillus thermoglucosidasius

AY608981

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

939-1405

94

Geobacillus caldoxylosilyticus

AF067651

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

925-1389

95

Geobacillus tepidamans

AY563003

G TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

872-1334

96

Geobacillus vulcani

AJ293805

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

920-1384

97

Filobacillus milosensis

AJ238042,

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

915-1375

98

Jeotgalibacillus alimentarius.

AF281158

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

911-1373

99

Jeotgalibacillus salarius

EU874389

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

910-1372

100

Jeotgalibacillus campisalis

AY190535

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

908-1370

101

Jeotgalibacillus marinus

AJ237708

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

918-1380

102

Ureibacillus thermosphaericus

AB101594

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

931-1392

103

Ureibacillus composti

DQ348071

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

929-1390

104

Ureibacillus thermophilus

DQ348072

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

931-1392

105

Ureibacillus suwonensis

AY850379

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%-

918-1379

106

Ureibacillus terrenus

AJ276403

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%-

900-1361

107

Lysinibacillus boronitolerans

AB199591

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

898-1360

108

Lysinibacillus xylanilyticus

FJ477040

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

826-1288

109

Lysinibacillus fusiformis

AJ310083

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

920-1382

110

Lysinibacillus sphaericus

AJ310084

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

920-1382

111

Lysinibacillus parviboronicapiens

AB300598

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

910-1372

112

Terribacillus goriensis

DQ519571

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

94%+

895-1355

113

Terribacillus saccharophilus

AB243845

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

94%+

922-1382

114

Terribacillus halophilus

AB243849

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

922-1382

115

Terribacillus aidingensis

FJ386524

CTGAAACTCAAAAG AATTGACG AATACGTTCCCGGGCCTT

95%+

922-1382

116

Bacillus massiliensis

AY677116

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

93%+

908-1370

117

Bacillus cecembensis

AM773821

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

93%+

931-1393

118

Bacillus odysseyi

AF526913

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

93%+

931-1393

119

Bacillus decisifrondis

DQ465405

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

85%+

843-1305

120

Bacillus psychrodurans

AJ277984

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

95%-

918-1380

121

Bacillus psychrotolerans

AJ277983

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

95%-

903-1365

122

Bacillus insolitus

AM980508

GAGGGGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

94%+

917-1378

123

Bacillus beijingensis

EF371374

AATACGTTCCCGGGTCTT CTGAAACTCAAAGGAATTGACG

96%-

929-1387

124

Bacillus ginsengi

EF371375

AATACGTTCCCGGGTCTT CTGAAACTCAAAGGAATTGACG

97%-

929-1387

125

Bacillus aquimaris

AF483625

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

99%+

910-1372

126

Bacillus vietnamensis

AB099708

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

98%+

903-1365

127

Bacillus marisflavi

AF483624

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

100%+

909-1371

128

Bacillus seohaeanensis

AY667495

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

98%+

872-1334

129

Bacillus mycoides

AB021192

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

97%+

907-1367

130

Bacillus weihenstephanensis

AB021199

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

97%+

925-1385

131

Bacillus thuringiensis

D16281

AATACGTTCCCGGGCCTT CTGAAACTCAAAGGAATTGACG

97%+

911-1371

132

Bacillus pseudomycoides

AF013121

CTGAAACTCAAAGGAT TTGACG AATACGTTCCCGGGCCTT

95%+

932-1392

133

Bacillus funiculus

AB049195

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

919-1379

134

Bacillus panaciterrae

AB245380

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

904-1364

135

Bacillus flexus

AB021185

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

923-1385

136

Bacillus megaterium

D16273

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

910-1372

137

Bacillus koreensis

AY667496

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

847-1309

138

Bacillus aerius

AJ831843

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

922-1382

139

Bacillus aerophilus

AJ831844

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

927-1387

140

Bacillus stratosphericus

AJ831841

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

927-1387

141

Bacillus sonorensis

AF302118

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

908-1368

142

Bacillus amyloliquefaciens

AB255669

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

909-1369

143

Bacillus siamensis

GQ281299

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%-

931-1352

144

Bacillus methylotrophicus

EU194897

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

898-1358

145

Bacillus subtilis subsp. subtilis

AJ276351

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

919-1379

146

Bacillus subtilis subsp. spizizenii

AF074970

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

907-1367

147

Bacillus vallismortis

AB021198

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

924-1384

148

Bacillus mojavensis

AB021191

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

920-1380

149

Bacillus atrophaeus

AB021181

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

909-1369

150

Bacillus pumilus

AY876289

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

879-1339

151

Bacillus safensis

AF234854

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

879-1339

152

Bacillus altitudinis

AJ831842

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

934-1394

153

Bacillus ginsengihumi

AB245378

TT GAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%-

914-1376

154

Bacillus acidiproducens

EF379274

TT GAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%-

879-1341

155

Bacillus acidicola

AF547209

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

99%+

934-1396

156

Bacillus oleronius

AY988598

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

934-1396

157

Bacillus sporothermodurans

U49078

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

904-1366

158

Bacillus carboniphilus

AB021182

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

910-1372

159

Bacillus chungangensis

FJ514932

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

890-1352

160

Bacillus endophyticus

AF295302

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

96%-

906-1362

161

Bacillus isabeliae

AM503357

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

912-1372

162

Bacillus shackletonii

AJ250318

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

909-1371

163

Bacillus circulans

AY043084

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

902-1364

164

Bacillus nealsonii

EU656111

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

928-1390

165

Bacillus korlensis

EU603328

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

889-1351

166

Bacillus siralis

AF071856

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

905-1367

167

Bacillus benzoevorans

X60611

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

95%-

931-1386

168

Bacillus firmus

D16268

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

907-1369

169

Bacillus infantis

AY904032

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

871-1333

170

Bacillus oceanisediminis

GQ292772

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

861-1323

171

Bacillus kribbensis

DQ280367

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%-

919-1381

172

Bacillus horneckiae

EU861362

TT GAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

796-1258

173

Bacillus badius

X77790

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%-

913-1370

174

Bacillus smithii

Z26935

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

924-1383

175

Bacillus aeolius

AJ504797

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%-

901-1361

176

Bacillus coagulans

AB271752

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%-

911-1373

177

Bacillus alveayuensis

AY605232

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

934-1396

178

Bacillus thermoamylovorans

L27478

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

92%+

930-1391

179

Bacillus fordii

AY443039

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

892-1354

180

Bacillus fortis

AY443038

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

927-1389

181

Bacillus farraginis

AY443036

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

838-1300

182

Bacillus galactosidilyticus

AJ535638

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

904-1367

183

Bacillus ruris

AJ535639

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%-

901-1363

184

Bacillus lentus

AB021189

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

928-1390

185

Bacillus novalis

AJ542512

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

908-1370

186

Bacillus vireti

AJ542509

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

908-1370

187

Bacillus bataviensis

AJ542508

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

908-1370

188

Bacillus drentensis

AJ542506

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

844-1306

189

Bacillus soli

AJ542513

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

908-1370

190

Bacillus fumarioli

AJ250056

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

909-1371

191

Bacillus niacini

AB021194

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

921-1383

192

Bacillus pocheonensis

AB245377

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

910-1372

193

Bacillus boroniphilus

AB198719

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

930-1392

194

Bacillus selenatarsenatis

AB262082

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

870-1332

195

Bacillus jeotgali

AF221061

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

908-1370

196

Bacillus thioparans

DQ371431

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

908-1370

197

Bacillus foraminis

AJ717382

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

922-1384

198

Bacillus canaveralius

DQ870688

CTGAAACTCAAAGGAATTGACG SEQUENEWAS SHORT

97%

887-1323

199

Bacillus infernus

U20385

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTN

95%+

921-1383

200

Bacillus methanolicus

AB112727

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

909-1372

201

Bacillus butanolivorans

EF206294

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

914-1380

202

Bacillus simplex

AJ439078

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

920-1379

203

Bacillus muralis

AJ316309

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

909-1371

204

Bacillus psychrosaccharolyticus

AB021195

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

900-1362

205

Bacillus asahii

AB109209

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

909-1373

206

Bacillus indicus

AJ583158

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

918-1381

207

Bacillus cibi

AY550276

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

896-1358

208

Bacillus idriensis

AY904033

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

889-1351

209

Bacillus niabensis

AY998119

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

904-1366

210

Bacillus fastidiosus

X60615

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

930-1386

211

Bacillus litoralis

AY608605

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

908-1370

212

Bacillus herbersteinensis

AJ781029

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

908-1370

213

Bacillus galliciensis

FM162181

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

908-1370

214

Bacillus alkalitelluris

AY829448

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

911-1373

215

Bacillus humi

AJ627210

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%

910-1372

216

Bacillus halmapalus

X76447

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

98%+

908-1370

217

Bacillus horikoshii

AB043865

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

929-1391

218

Bacillus cohnii

X76437

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

910-1372

219

Bacillus acidiceler

DQ374637

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

916-1376

220

Bacillus luciferensis

AJ419629

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

909-1369

221

Bacillus azotoformans

AB363732

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

909-1370

222

Bacillus taeanensis

AY603978

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

917-1378

223

Bacillus macauensis

AY373018

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

88-1350

224

Bacillus rigui

EU939689

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%-

903-1365

225

Bacillus solisalsi

EU046268

CTGAAACTCAAAA GGAATTGACG AATACGTTCCCGGGCCTT

95%-

887-1349

226

Bacillus gelatini

AJ551329

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

909-1371

227

Bacillus arsenicus

AJ606700

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%-

928-1390

228

Bacillus barbaricus

AJ422145

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%-

882-1342

229

Bacillus algicola

AY228462

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

931-1393

230

Bacillus hwajinpoensis

AF541966

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

909-1371

231

Bacillus decolorationis

AJ315075

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

909-1371

232

Bacillus okuhidensis

AB047684

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

874-1335

233

Bacillus lehensis

AY793550

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

95%-

939-1395

234

Bacillus oshimensis

AB188090

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

95%-

937-1393

235

Bacillus patagoniensis

AY258614

AATACGTTCCCGGGTC TT T TGAAACTCAAAGGAATTGACG

95%-

913-1369

236

Bacillus clausii

X76440

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

95%-

913-1369

237

Bacillus gibsonii

X76446

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

912-1372

238

Bacillus murimartini

AJ316316

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

913-1373

239

Bacillus plakortidis

AJ880003

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

906-1366

240

Bacillus pseudalcaliphilus

X76449

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

909-1371

241

Bacillus trypoxylicola

AB434284

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

911-1373

242

Bacillus alcalophilus

X76436

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

909-1371

243

Bacillus bogoriensis

AY376312

CTGAAACTCAAAGGAATTGAGC AATACGTTCCCGGGCCTT

97%+

911-1374

244

Bacillus akibai

AB043858

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

950-1411

245

Bacillus krulwichiae

AB086897

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

912-1374

246

Bacillus okhensis

DQ026060

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

916-1378

247

Bacillus wakoensis

AB043851

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

930-1392

248

Bacillus hemicellulosilyticus

AB043846

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

940-1402

249

Bacillus macyae

AY032601cpf

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

916-1378

250

Bacillus alkalinitrilicus

EF422411

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

919-1381

251

Bacillus pseudofirmus

X76439

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

97%+

910-1372

252

Bacillus qingdaonensis

DQ115802

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

913-1375

253

Bacillus halochares

AM982516

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%-

881-1343

254

Bacillus aidingensis

DQ504377

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%-

946-1407

255

Bacillus salarius

AY667494

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

858-1320

256

Bacillus persepolensis

FM244839

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

940-1402

257

Bacillus agaradhaerens

X76445

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGT CTT

96%+

925-1385

258

Bacillus neizhouensis

EU925618

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

96%+

905-1367

259

Bacillus beveridgei

FJ825145

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

944-1409

260

Bacillus chagannorensis

AM492159

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%+

945-1407

261

Bacillus saliphilus

AJ493660

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

919-1381

262

Bacillus aurantiacus

AJ605773

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

929-1381

263

Bacillus vedderi

Z48306

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%-

905-1367

264

Bacillus cellulosilyticus

AB043852

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%-

924-1386

265

Bacillus clarkii

X76444

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

95%+

926-1328

266

Bacillus polygoni

AB292819

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

94%-

945-1408

267

Bacillus horti

D87035

CTGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

93%+

923-1378

268

Bacillus mannanilyticus

AB043864

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

96%-

955-1413

Actinobacteria (High GC content gram positive bacteria)

269

Corynebacterium diphtheriae

X84248

CTA AAACTCAAAGGAATTGACG AATACGTNCCCGGGCCTT

83%-

880-1341

270

Mycobacterium tuberculosis

X58890

CTAAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

85%-

1541-2002

271

Nocardia asteroides

AF430019

CTA AAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

84%-

875-1376

272

Streptomyces lavendulae subsp. lavendulae

D85116

CTA AAACTCANAGGAATTGACG AATACGTTCCCGGGCCTT

81%-

893-1361

Low GC content Firmicutes (gram +ve)

273

Staphylococcus chromogenes

D83360

AATACGTTCCCGGGTC TT CTGAAACTCAAAGGAATTGACG

92%+

913-1371

274

Streptococcus pyogenes

AB002521

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

89%+

890-1350

275

Enterococcus faecalis

AB012212

T TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

91%+

939-1395

276

Clostridium populeti

X71853

A TGAAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

86%-

903-1359

277

Listeria monocytogenes.

X56153

AATACGTTCCCGGGCCTN T TGAAACTCAAAGGAATTGACG

94%+

936-1392

Alpha proteobacteria

278

Rhizobium leguminosarum

U29386

TTA AAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

86%-

913-1371

279

Azospirillum lipoferum

Z29619

TTA AAACTCAAAGGAATTGACG AATACGTTCCCGGGCCTT

84%-

845-1305

280

Acetobacterium woodii

X96954

T TGAAACTCAAAGGAATTGACG AATG CGTTCCCGGGTCTT

90%-

840-1305

Beta proteobacteria

281

Burkholderia cepacia

U96927

AATACGTTCCCGGGTC TT TTA AAACTCAAAGGAATTGACG

82%-

870-1322

282

Bordetella pertussis

U04950

TTA AAACTCAAAGGAATTGACG AATACGTTCCCGGGTC TT

81%-

922-1375

Gamma proteobacteria

283

Pseudomonas aeruginosa

X06684

AATACGTC CCCGGGCCTT TTA AAACTCAAATGAATTGACG

86%-

923-1384

284

Escherichia coli

X80725

TTA AAACTCAAATGAATTGACG AATACGTTCCCGGGTC TT

83%-

921-1379

285

Klebsiella pneumoniae subsp. pneumoniae

X87276

TT AAAACTCAAATGAATTGACG AATACGTTCCCGGGTC TT

82%-

920-1381

286

Shigella dysenteriae

X96966

TTA AAACTCAAATGAATTGACG AATACGTTCCCGGGTC TT

83%-

908-1362