Impaired of a non-DNA dependent methylation status decides the fat decision of bone marrow-derived C3H10T1/2 stem cell

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Table 1 Effects various concentrations of Adox on content of SAM, SAH and SAM: SAH ratios for 48 h in C3H10T1/2 stem cells ¹

Treatment	SAH²(nmol/L)	SAM (nmol/L)	SAM: SAH ratio
Control	1.6 ± 0.48^a	28 ± 0.41^b	17.5 ± 0.28^c
DSMO + OB	1.7 ± 0.38^a	31 ± 0.32^b	18.2 ± 0.26^c
Adox + OB
10 μM	1.9 ± 0.12^a	35 ± 0.25^c	18.4 ± 0.16b^a
25 μM	2.66 ± 0.14^b	33 ± 0.29^a,b	12.4 ± 0.14^c
50 μM	3.99 ± 0.19^c	34 ± 0.23^b	8.5 ± 0.18^b,c

¹Cells in the control condition were only exposed to control medium without any osteogenic supplements, the other cells were cultured in OB medium treated with (DMSO) as a solvent control (the final concentration of DMSO was 0.2%). Cells in OB (osteogenic medium) exposed to OB medium without Adox treatment. The other cells were cultured in OB medium containing different concentrations of Adox (5-50 μM). ²Values are means ± SD of triplicate assays. Data in the column with different superscript letters are significantly different (P < 0.05).