Fucci2 probes of mES cell cycle on MEF and E-cad-Fc. A fluorescent probe that labels individual G1 phase nuclei in red and S/G2/M phase nuclei in green (A). Schematic representation of Fucci2 mES cells seeded on MEF and E-cad-Fc (B). Real imaging of Fucci2 mES cells on MEF in colonies formation and E-cad-Fc with singly scattered cells using confocal microscope FV1000D (C). Scale bar: 10 μm.