Table 2 Recoveries of cefuroxime, cefatoxime and cefradine using SPE at various concentrations and with different elution solvents

Drugs optimized individually

Cefuroxime

10

2

HLB

80% MeOH

5

Cefatoxime

10

2

HLB

80% MeOH

24

Cefatoxime

10

2

HLB

100% MeOH

79

Cefuroxime

10

2

HLB

100% MeOH

47

Cefradine

20

C-18

55:45 MeOH:0.05% formic acid

90

Recovery using mixture of drugs

Cefatoxime

50

2

HLB

100% MeOH

26

Cefatoxime

50

2

HLB

2 mL MeOH with 8 mL acetone

53

Cefatoxime

50

2

HLB

8 mL acetone

85

Cefuroxime

50

2

HLB

100% MeOH

29

Cefuroxime

50

2

HLB

8 mL acetne

73

Cefuroxime

50

2

HLB

8 mL acetone

77

Cefradine

50

2

HLB

100% MeOH

00

Cefradine

50

2

C-18

55:45 MeOH:0.05% formic acid

78

Recovery with HLB and C-18 in series with mixture of drugs

Cefatoxime

50

2

Mixed mode

8 mL acetone

104

Cefuroxime

50

2

Mixed mode

8 mL acetone

44

Cefradine

100

2

Mixed mode

55:45 MeOH:0.05% formic acid

55

Recoveries at 1 μg mL^-1 concentrations

Cefatoxime

2

Mixed mode

8 mL acetone

95

Cefurxime

2

Mixed mode

8 mL acetone

83

Cefradine

2

Mixed mode

55:45 MeOH:0.05% formic acid

80