Figure 6
The effect of p31 comet expression levels on drug resistance cancer cell lines. (a) Western blotting analysis of p31comet expression levels in cancer cell lines. Each cell extract was subject to western blotting with anti-p31comet antibody, anti-Mad2 and anti-Cdc20 antibodies to monitor SAC components, anti-EB1 and anti-α-Tubulin antibodies to monitor microtubule components, and anti-APC2 antibody for a loading control. The quantitative p31comet/Mad2 expression level ratios were shown. (b) Cell viability analysis of cancer cell lines against taxol. Each cancer cell lines with different levels of p31comet was treated with the indicated concentration of taxol and subject to trypan blue exclusion to counte live cells at 2 days. Two hundred cells were scored at each point.