Skip to main content
SpringerPlus
Figure 2 | SpringerPlus

Figure 2

From: p31comet inactivates the chemically induced Mad2-dependent spindle assembly checkpoint and leads to resistance to anti-mitotic drugs

Figure 2

The effect of p31 comet overexpression on anti-mitotic drug-treated cells. (a) FACS analysis of p31comet-overexpressing cells treated with anti-mitotic drugs (nocodazole, taxol, and monastrol). Cells were infected with EGFP or EGFP-p31comet adenovirus for 24 h, washed with fresh medium twice, and treated with anti-mitotic drugs at the indicated concentration for 24 h. These cells were prepared for FACS analysis. A population of >4 N DNA content in the cells was shown. (b) Western blotting analysis of p31comet-overexpressing cells treated with anti-mitotic drugs. Drug-treated cell extracts were subject to western blotting with anti-Cdc27 and anti-Securin antibodies to monitor the cell cycle status, anti-GFP antibody for EGFP and EGFP-p31comet, and anti-Mad2 antibody for a loading control.

Back to article page