Table 2 Radiation impact on Escherichia coli strains assessed by clonogenic (surviving fraction) and colorimetric (resazurin) assays a
From: Resazurin-based assay for screening bacteria for radiation sensitivity
E. coli K-12 strain (DNA repair defect) | X-irradiation (250 Gy) | UV-irradiation (100 J m-2) | ||
|---|---|---|---|---|
| Â | Surviving fraction | Resazurin A492 | Surviving fraction | Resazurin A492 |
SR749 (parental, control) | 2.9 (±0.7) e-1 | 0.7 (±0.04) | 6.2 (±0.4) e-1 | 0.7 (±0.02) |
SR1159 (recB21) | 3.9 (±0.7) e-7 | 2.6 (± 0.04) | 1.0 (±0.0) e-2 | 1.6 (±0.2) |
SR1165 (umuC122::Tn5) | 2.4 (±0.5) e-1 | 0.7 (±0.02) | 3.9 (±1.3) e-1 | 0.9 (±0.09) |
SR1187 (radC102) | 2.0 (±1.0) e-2 | 2.8 (±0.6) | 1.5 (±0.4) e-1 | 2.4 (±0.03) |
SR1252 (polA5) | 4.8 (±4.3) e-6 | 3.1 (±0.04) | 6.8 (±2.5) e-4 | 3.2 (±0.2) |
SR1277 (uvrD254::Tn5) | 4.8 (±4.5) e-3 | 1.9 (±0.20) | 1.3 (±0.5) e-1 | 2.1 (±0.5) |
SR1279 (lexA101) | 8.5 (±0.4) e-7 | 1.8 (±0.09) | 2.0 (±0.0) e-2 | 1.9 (±0.2) |
SR1467 (recA srl301::Tn10) | 3.4 (±0.4) e-7 | 2.8 (±0.2) | 2.3 (±0.9) e-5 | 3.2 (±0.3) |
SR1553 (recN262) | 1.0 (±1.3) e-3 | 1.5 (±0.05) | 4.3 (±0.9) e-1 | 1.0 (±0.1) |
SR1643 (recC22) | 6.7 (±1.8) e-7 | 2.1 (±0.2) | 2.0 (±2.0) e-2 | 2.4 (±0.1) |
SR1663 (recJ284::Tn10) | 9.0 (±2.0) e-2 | 0.9 (±0.07) | 4.3 (±0.4) e-1 | 1.0 (±0.1) |
SR2384 (ruvA59::Tn10) | 2.0 (±0.3) e-4 | 2.0 (±0.3) | 1.8 (±0.6) e-3 | 2.5 (±0.6) |
SR2385 (ruvB60::Tn10) | 2.1 (±0.09) e-4 | 1.9 (±0.3) | 1.7 (±0.4) e-3 | 2.1 (±0.3) |
SR2603 (ΔruvC64::kan) | 1.4 (±0.6) e-3 | 1.9 (±0.2) | 8.2 (±3.1) e-3 | 1.5 (±0.1) |
SR2604 (recG258::kan) | 7.6 (±4.1) e-3 | 1.3 (±0.3) | 6.0 (±2.0) e-2 | 1.1 (±0.2) |
SR2666 (recF332::Tn3) | 1.1 (±0.4) e-5 | 2.8 (±0.02) | 5.0 (±2.0) e-2 | 2.8 (±0.02) |
SR2877 (radA110::kan) | 6.0 (±2.0) e-2 | 0.9 (±0.2) | 2.3 (±0.6) e-1 | 1.0 (±0.06) |