Resazurin-based assay for screening bacteria for radiation sensitivity

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Table 1 Escherichia coli K-12 strains used in this study ^a

Strains	Genotype	Source, Reference
SR749	parental, control	AB1157, E. coli Genetic Stock Center
SR1159	recB21	NJS Lab strain
SR1165	umuC122::Tn5	NJS Lab strain
SR1187	radC102	I Felszenswalb
SR1252	polA5	NJS Lab strain
SR1277	uvrD254::Tn5	NJS Lab strain
SR1279	lexA101	NJS Lab strain
SR1467	recA srl301::Tn10	NJS Lab strain
SR1553	recN262	NJS Lab strain
SR1643	recC22	NJS Lab strain
SR1663	recJ284::Tn10	NJS Lab strain
SR2384	ruvA59::Tn10	NJS Lab strain
SR2385	ruvB60::Tn10	NJS Lab strain
SR2603	ΔruvC64::kan	RG Lloyd
SR2604	recG258::kan	RG Lloyd
SR2666	recF332::Tn3	NJS Lab strain
SR2877	radA110::kan	NJS Lab strain

^a All strains were derived from the DNA repair proficient E. coli K-12 AB1157 strain (SR749), and carry the following mutations: argE3(oc), hisG4(oc), leuB6(amIII), Δ(gpt-proA)62, thr-1, thi-1, ara-14, galK2, lacY1, mtl-1, xyl-5, tsx-33, rfbD1, mgl-51, rpsL31, supE44(amSuII), rac, F^-, λ^-. Genetic nomenclature has been described (Berlyn 1998).