Figure 4
Antifungal activity of fractions from DEAE-sepharose Fast Flow and SephadexG-100 chromatographies toward Fusarium oxysporum. A: control (10 μl 20 mmol/L Na2HPO 4/NaH2PO4 buffer (PB buffer), pH 6.8); B: fraction III from DEAE-sepharose Fast Flow column chromatography; C: 20 μg antifungal protein (fraction P1) in 10 μl PB buffer; D: 12 μg antifungal protein (fraction P1) in 10 μl PB buffer.