Figure 8

BPA induces the cell migration of androgen-independent prostate cancer cells. (A) Cell migration was studied via the in vitro wound-healing assay. The PC-3 cells grown to confluent monolayers were incubated for 48 h in CS-RPMI, complemented or not by BPA at different concentrations. The cells were then wounded using a sterile tip and incubated in the corresponding media for an additional 15 h before the microscopic examinations of the wound-healing of the cells. Wound-healing was examined 1 and 15 hours after scratching, in at least 3 different previously identified (Blue line) fields per scratch and representative figures for each condition are presented here. (B) Western blot analysis of whole cell lysate proteins of Orai1, in PC-3 cells treated with BPA at different concentrations. The figure is a representative experiment showing the variation of Orai1 protein under BPA treatments. β-actin was used as a sample loading control. The fold variation of the Orai1 protein in different samples normalized to β-actin expression is shown under the panel. Results shown are representative of at least three independent experiments.