Figure 7

BPA-induced SOCE is involved in prostate cancer cells migration. Cell migration was measured via the Transwell chamber assay. (A) Following the 48 h 10 nM BPA treatment for, LNCaP cells were trypsinized and equal numbers of live cells were plated into inserts and allowed to migrate for 24h in the presence or absence of BPA 10 nM, with or without the presence of a Ca²⁺ chelator EGTA (1 mM) or the SOCE inhibitors (SKF, 20 μM; BTP-2, 2 μM). Cells in the insert (non-migrated cells) were then eliminated by scraping and migrated cells were fixed and their nuclei were stained by Hoechst 33342 dye (Blue) and examined under fluorescence microscopy. At least ten different fields per condition were examined and the cells counted. Experiments were performed in duplicate and repeated at least twice. (B) Relative migration (%) ± SE (Bar) is shown for the control condition (CTL-ST), where the cells were incubated in CS-RPMI alone, and for BPA (10 nM) in the presence or absence of the EGTA or SOCE inhibitors. *, P < 0.001 relative to CTL condition; ** P < 0.001 BPA 10 nM versus BPA 10 nM + inhibitors.