Figure 3

Effects of BPA on basal calcium and Store-operated Ca ²⁺ (SOC) entry (SOCE) in LNCaP C4.2 cells. (A) Cells were grown on glass coverslips and cultured in complete medium and then the direct effects of BPA at different concentrations on [Ca²⁺]_i were studied by calcium imaging. (B) Cells grown on glass coverslips and cultured in CS-RPMI alone (CTL), or containing BPA at 1 nM BPA for 48h and then processed by calcium imaging to estimate the amplitude of SOCE. For Ca²⁺ recording, cells were treated with 200 nM thapsigargin (TG) in Ca²⁺-free bath solution (0 Ca²⁺) and exposed to 2 mM extracellular Ca²⁺ (2 Ca²⁺) as indicated. Each experiment was repeated at least 3 times in duplicate in different cell cultures on a field of 25–40 cells and representative experiments performed on 50–80 cells as mean ± S.E. are presented. A quantification of the SOCE for experiment (B) is presented in (C) (mean ± S.E., n = 50–80 cells), *P < 0.01. The TG application is shown by an arrow and extracellular Ca2+ increases from 0 (0 Ca²⁺) to 2 mM (2 Ca²⁺) are marked by horizontal bars. The graphs represent the mean peak amplitudes of calcium mobilization and SOCE ± S.E. of at least three independent experiments and are expressed as the percentage above control value.