Figure 1

BPA induces prostate cancer cells migration. Cell migration was measured via the Transwell chamber assay. (A) Following the 48 h BPA treatments, LNCaP cells were trypsinized and equal numbers of live cells were plated into inserts and allowed to migrate for 24 h. Cells in the insert (non-migrated cells) were eliminated by scraping and migrated cells were fixed and their nuclei were stained by Hoechst 33342 dye (Blue) and examined under fluorescence microscopy. At least ten different fields per condition were examined and cells were then counted. Experiments were performed in duplicate and repeated at least twice. (B) Relative migration (%) ± S.E. (Bar) is shown for the indicated BPA concentrations and the control condition (CTL-ST), where the cells were incubated in CS-RPMI alone. *, P < 0.001 relative to CTL condition.