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Table 1 Effect of culture conditions for production of extracellular protease from B. licheniformis P003 in shake-flask cultivation

From: Optimization and partial characterization of culture conditions for the production of alkaline protease from Bacillus licheniformis P003

Culture condition

Protease activity (U/ml)

Relative activity (%)

Protease activity (U/mg)

Total soluble protein (mg/ml)

Mean±SE

Mean±SE

Mean±SE

Initial pH

    

8.0

39.01±0.190

43.68

43.72±1.22

0.889±0.021

8.5

81.28±0.199

91.02

86.13±2.12

0.944±0.021

9.0

89.30±0.661

100.00

104.10±1.53

0.858±0.016

9.5

79.38±0.143

88.89

83.81±1.51

0.947±0.016

10.0

80.55±0.194

90..20

94.41±1.45

0.853±0.015

10.5

74.62±0.562

83.56

90.70±5.48

0.825±0.057

11.0

10.91±0.185

12.22

14.74±0.09

0.74±0.010

Incubation temperature (°C)

    

32

39.08±0.096

72.21

35.33±0.11

1.106±0.006

35

45.10±0.070

83.33

40.60±0.40

1.112±0.013

37

48.71±0.026

90.00

42.42±0.22

1.143±0.014

40

50.19±0.053

92.74

43.16±0.49

1.155±0.023

42

54.12±0.056

100.00

43.10±0.27

1.255±0.007

45

43.90±0.098

81.12

35.17±0.20

1.247±0.010

Incubation period (hr)

    

24

9.62±0.062

20.00

7.80±0.07

1.231±0.008

48

48.10±0.062

100.00

40.74±0.17

1.178±0.008

72

36.68±0.046

76.26

30.51±0.12

1.200±0.007

96

34.27±0.072

71.25

29.92±0.11

1.143±0.007

120

22.85±0.046

47.51

20.00±0.04

1.141±0.005

Inoculums volume (%, ml)

    

0.5

13.89±0.026

25.95

16.31±0.09

0.849±0.008

1.0

35.48±0.026

66.29

41.51±0.20

0.853±0.006

1.5

36.68±0.053

68.54

43.12±0.17

0.848±0.008

2.0

53.52±0.030

100.00

58.54±0.37

0.915±0.006

2.5

19.24±0.036

35.95

27.15±0.38

0.709±0.009

3.0

13.23±0.026

24.72

19.23±0.17

0.689±0.008

3.5

12.38±0.035

53.03

23.31±0.39

0.531±0.010

  1. Data represent as mean ± standard error (SE) for three replicates.