Table 1 Sequences of SCAR primers, PCR condition and product size
From: Molecular cloning and development of RAPD-SCAR markers for Dimocarpus longan variety authentication
SCAR | 5′-primer | Sequence (5′-3′) | 3′-primer | Sequence (5′-3′) | Size (bp) | Tm (°C) |
|---|---|---|---|---|---|---|
LY2-1 | LY2-1 L | AACTGGAAGTCCCTGGTCCT | LY2-1R | ACAAGAGGCCCCAGTAAGGT | 350 | 60 |
LY4-7 | LY4-7 L | GGCGCCGGTATACTTTGTAA | LY4-7R | CTCGTAAGAGGATCCGTCCA | 367 | 60 |
LY4-8 | LY4-8 L | CCCCATCTGGTTGTAGCACT | LY4-8R | AGCCAGCTCAACCAACTCAT | 358 | 60 |
LY5-2 | LY5-2 L | TTTTAATGTTGGGCATTTGG | LY5-2R | GCTAACCGAGATCCACTAACG | 250 | 60 |