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Figure 5 | SpringerPlus

Figure 5

From: Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens

Figure 5

Protease resistance of the antigen recognized by MAb 10A5. Samples of the 25/45 fraction (20 ÎĽg) removed after 0, 2, 24, and 48 hours of digestion with trypsin or proteinase K (Prot K) were run on duplicate 18% resolving gels (10 ÎĽg/lane). (A) Digests from one gel were probed with MAb 10A5 by immunoblot analysis. (B) The duplicate gel was stained with Coomassie Blue R-250. Arrows mark trypsin and proteinase K at 23.8 kD and 28.9 kD, respectively.

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