Mutational pressure dictates synonymous codon usage in freshwater unicellular α - cyanobacterial descendant Paulinella chromatophora and β - cyanobacterium Synechococcus elongatus PCC6301

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Table 6 Spearmen’s rank correlation analysis between COA axes and codon usage indices

Axes	A₃	T₃	G₃	C₃	GC₃	ENC	CAI	Gravy score	Aromaticity	Length of CDS
Axis 1	−0.434**	−0.094	0.221**	0.559**	0.565**	0.345**	−0.360**	−0.064	−0.081	−0.140**
Axis 2	−0.159**	0.118**	0.092	−0.014	0.041	−0.072	0.104	−0.045	−0.028	0.006
Axis 3	0.008	0.016	0.057	−0.057	−0.015	0.044	0.063	−0.023	−0.024	0.065
Axis 4	0.173**	−0.404**	0.125	0.167**	0.187**	0.343**	−0.258**	0.043	−0.028	−0.074
Axis 5	−0.027	0.060	−0.031	−0.006	−0.035	−0.031	−0.022	−0.075	−0.030	−0.096

Correlation analysis between five different axes of COA and various codon usage indices of 786 PCG in the chromatophore genome of P. chromatophora.
Analysis was made using Spearman’s rank correlation method.
**Significant at p ≤ 0.001 (one tailed).