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Table 4 Primers used for amplifying 16S ribosomal RNA gene sequences from algal samples from Sacramento-San Joaquin Delta and Clear Lake

From: Identification of harmful cyanobacteria in the Sacramento-San Joaquin Delta and Clear Lake, California by DNA barcoding

Target (size)

Primer

Sequence (5′ - 3′)

Reference

16S rDNA-ITS (1.5- 2 kb)

pA

AGAGTTTGATCCTGGCTCAG

Edwards et al. 1989

 

B23S

CTTCGCCTCTGTGTGCCTAGGT

Lepere et al. 2000

16S rDNA (1.5 kb)

CYA108F

ACGGGTGAGTAACRCGTRA

Urbach et al. 2001

 

CYA16S SCYR

CTTCAYGYAGGCGAGTTGCAGC

16S rDNA for sequencing

AlgaeIDSqF4

CGTGCCAGCAGCCGCGGTAATACG

This study