Identification of thermo tolerant lactic acid bacteria isolated from silage prepared in the hot and humid climate of Southwestern Japan

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Table 2 Characteristics of isolated strains

Strain	NGRI 0001	NGRI 0101	NGRI 0110	NGRI 0130	NGRI 0225	NGRI 0304	NGRI 0305	NGRI 0315	NGRI 0404	NGRI 0510*	NGRI 0524	NGRI 0529
Shape	Rods	Rods	Rods	Rods	Rods	Cocci	Cocci	Rods	Rods	Cocci	Rods	Rods
Gas from glucose	-	-	-	+	-	-	+	-	-	-	-	-
Growth at 15°C	+	+	+	+	+	+	+	+	+	+	+	+
Growth at 45°C	+	-	+	+	-	-	-	-	-	-	-	-
Fermentation type	Homo	Homo	Homo	Hetero	Homo	Homo	Hetero	Homo	Homo	Homo	Homo	Homo
Lactate formed	DL	DL	L	DL	DL	L	DL	DL	DL	DL	DL	DL
Peptidoglycan type	A₂pm	A₂pm	Non- A₂pm	Non- A₂pm	A₂pm	Non- A₂pm	Non- A₂pm	A₂pm	A₂pm	A₂pm	A₂pm	A₂pm
Acid from:
Na-Gluconate	+	+	+	+	+	+	+	+	+	+	+	+
L- Arabinose	+	-	+	+	-	+	+	+	+	+	+	+
Lactose	+	+	+	-	+	-	-	+	+	-	+	+
Galactose	+	+	+	-	+	+	+	+	+	+	+	+
Sucrose	+	+	+	-	+	+	+	+	+	-	+	+
Mannose	+	+	+	-	+	+	+	+	+	+	+	+
Fructose	+	+	+	-	+	+	+	+	+	+	+	+
Soluble starch	-	-	-	-	-	-	-	-	-	-	-	-
D-Xylose	+	-	-	+	-	-	+	+	+	+	+	+
D-Ribose	+	+	+	+	+	+	+	+	+	+	+	+
Mannitol	+	+	+	-	+	-	+	+	+	+	+	+
Sorbitol	+	+	+	-	+	+	-	+	+	+	+	+
Rhamnose	+	-	+	-	-	+	-	+	-	+	+	-
Trehalose	+	+	+	-	+	+	+	+	+	-	+	+
Raffinose	+	+	-	+	+	+	+	+	+	-	+	+
DNA G+C content (%)	45.1	45.3	47.3	46.2	45.1	39.7	39.0	44.4	45.1	41.0	46.1	44.0