Table 1 Primers used in this study
Primer | Sequence (5’ to 3’) | Use | Specificity or target | Reference or source |
|---|---|---|---|---|
HDA1 | ACTCCTACGGGAGGCAGCAGT | PCR | V2-V3 region of 16SrRNA | (Tannock et al. 1999) |
HDA2 | GTATTACCGCGGCTGCTGGCAC | PCR | V2-V3 region of 16SrRNA | (Tannock et al. 1999) |
16A-1 | GAATCGCTAGTAATCG | PCR | 16S-23S intergeneic spacer region | (Tannock et al. 1999) |
23B-1 | GGGTTCCCCCATTCGGA | PCR | 16S-23S intergeneic spacer region | (Tannock et al. 1999) |
M13 Primer M3 | GTAAA ACGACGGCCAGT | DNA sequence | Plasmid vector pTA2 | TaKaRa Bio |
M13 Primer RV | CAGGA AACAGCTATGAC | DNA Sequence | Plasmid vector pTA2 | TaKaRa Bio |
AP-A-2 | TGGATTGGTC | RAPD | Â | This study |
AP-A-6 | AAACTCCGTC | RAPD | Â | This study |
AP-A-8 | TGGTAAAGGG | RAPD | Â | This study |
AP-A-19 | GATCATAGCC | RAPD | Â | This study |
AP-A-23 | GATCTGACTG | RAPD | Â | This study |