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Table 1 Metal dissociation constants of ArgE H355A and WT ArgE determined by ITC and UV–vis absorption titration

From: Identification of a Histidine Metal Ligand in the argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli

Enzyme	Metal	Method	K _d1(μM)	K _d2(μM)
WT ArgE^a	Zn(II)	ITC	2.7	51
H355A ArgE	Zn(II)	ITC	39	330
WT ArgE^a	Co(II)	ITC	0.4	153
H355A ArgE	Co(II)	UV–vis Titration	1.6	ND^b

^aPreviously reported (ref. (McGregor et al. 2005)).
^bNone detected.

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