Figure 6From: Identification of a Histidine Metal Ligand in the argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli (Top) ITC titration data for a 70 μM solution of apo- H355A ArgE with a 2.5 mM solution of Zn(II). (Bottom) Fit of the ITC data for H355A ArgE after subtracting the heat of dilution. (Reaction conditions: 25°C in 50 mM HEPES buffer, pH 7.5, and 150 mM KCl).Back to article page